Pavel Jandera was a world-leading analytical chemist who devoted his entire professional life to research in the field of high-performance liquid chromatography. During his scientific career, he worked at the Department of Analytical Chemistry at the University of Pardubice, Czech Republic. His greatest contribution to the field of liquid chromatography was the introduction of a comprehensive theory of liquid chromatography with programmed elution conditions. He was also involved in the research of gradient elution techniques in preparative chromatography, modeling of retention and selectivity in various phase systems, preparation of organic monolithic microcolumns, and, last but not least, in the development of theory and practical applications of two-dimensional liquid chromatography, mainly in the comprehensive form. In this review article, we have tried to capture the highlights of his scientific career and provide the readers with a detailed overview of Pavel Jandera's contribution to the evolution of separation sciences.

Department of Analytical Chemistry Faculty of Chemical Technology University of Pardubice Pardubice Czech Republic

Department of Chemistry Faculty of Science Masaryk University Brno Czech Republic

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Jandera P, Churáček J. Ion-exchange chromatography of carboxylic-acids. J Chromatogr. 1973;86:351-421.

Jandera P, Churáček J. Ion-exchange chromatography of sulfur compounds, phenols, phosphorus compounds and esters of carboxylic acids. J Chromatogr. 1973;86:423-49.

Jandera P, Churáček J. Ion-exchange chromatography of nitrogen compounds. J Chromatogr. 1974;98:1-54.

Jandera P, Colin H, Guiochon G. Interaction indexes for prediction of retention in reversed-phase liquid-chromatography. Anal Chem. 1982;54:435-41.

Colin H, Guiochon G, Jandera P. Interaction indexes and solvent effects in reversed-phase liquid-chromatography. Anal Chem. 1983;55:442-6.

Jandera P. Correlation of retention and selectivity of separation in reversed-phase high-performance liquid-chromatography with interaction indexes and with lipophilic and polar structural indexes. J Chromatogr A. 1993;656:437-67.

Holčapek M, Jandera P. Coupling of liquid chromatography and mass spectrometry. Chem Listy. 1998;92:278-86.

Holčapek M, Jandera P, Fischer J. Analysis of acylglycerols and methyl esters of fatty acids in vegetable oils and in biodiesel. Crit Rev Anal Chem. 2001;31:53-6.

Jandera P, Blomberg LG, Lundanes E. Controlling the retention in capillary LC with solvents, temperature, and electric fields. J Sep Sci. 2004;27:1402-18.

Jandera P, Novotná K. Characterization of high-pressure liquid chromatography columns using chromatographic methods. Anal Lett. 2006;39:2095-152.

Klodzinska E, Moravcová D, Jandera P, Buszewski B. Monolithic continuous beds as a new generation of stationary phase for chromatographic and electro-driven separations. J Chromatogr A. 2006;1109:51-9.

Jandera P. Column selectivity for two-dimensional liquid chromatography. J Sep Sci. 2006;29:1763-83.

Jandera P. Can the theory of gradient liquid chromatography be useful in solving practical problems? J Chromatogr A. 2006;1126:195-218.

Jandera P. Selection of separation conditions for HPLC and HPLC-MS of aromatic sulphonic acids and acid azo dyes. J Liq Chromatogr Relat Technol. 2007;30:2349-67.

Jandera P. Stationary phases for hydrophilic interaction chromatography, their characterization and implementation into multidimensional chromatography concepts. J Sep Sci. 2008;31:1421-37.

Urban J, Jandera P. Polymethacrylate monolithic columns for capillary liquid chromatography. J Sep Sci. 2008;31:2521-40.

Lasáková M, Jandera P. Molecularly imprinted polymers and their application in solid phase extraction. J Sep Sci. 2009;32:1036-42.

Jandera P. Stationary and mobile phases in hydrophilic interaction chromatography: a review. Anal Chim Acta. 2011;692:1-25.

Jandera P. Comprehensive two-dimensional liquid chromatography - practical impacts of theoretical considerations. A review. Cent Eur J Chem. 2012;10:844-75.

Jandera P. Programmed elution in comprehensive two-dimensional liquid chromatography. J Chromatogr A. 2012;1255:112-29.

Urban J, Jandera P. Recent advances in the design of organic polymer monoliths for reversed-phase and hydrophilic interaction chromatography separations of small molecules. Anal Bioanal Chem. 2013;405:2123-31.

Jandera P. Advances in the development of organic polymer monolithic columns and their applications in food analysis-a review. J Chromatogr A. 2013;1313:37-53.

Jandera P, Hájek T, Staňková M. Monolithic and core-shell columns in comprehensive two-dimensional HPLC: a review. Anal Bioanal Chem. 2015;407:139-51.

Jandera P, Janás P. Recent advances in stationary phases and understanding of retention in hydrophilic interaction chromatography. A review. Anal Chim Acta. 2017;967:12-32.

Jandera P, Hájek T. Mobile phase effects on the retention on polar columns with special attention to the dual hydrophilic interaction-reversed-phase liquid chromatography mechanism, a review. J Sep Sci. 2018;41:145-62.

Jandera P, Hájek T. Dual-mode hydrophilic interaction normal phase and reversed-phase liquid chromatography of polar compounds on a single column. J Sep Sci. 2019;43:70-86.

Jandera, P. 50 years of high-performance liquid chromatography: the contribution of the department of analytical chemistry in Pardubice. Sci Papers Univ Pardubice. 2020;26:5-66.

Jandera P. In: Pól J, Volný M, Nováková L, Douša M, Havliš J, Bednář P, editors. Počátky a Historie Československé Kapalinové Chromatografie. Prague: Česká společnost pro hmotnostní spektrometrii; 2015. p. 70-134.

Horváth CG, Preiss BA, Lipsky SR. Fast liquid chromatography - an investigation of operating parameters and separation of nucleotides on pellicular ion exchangers. Anal Chem. 1967;39:1422-8.

Jandera P, Churáček J, Gradient elution in column liquid chromatography. theory and practice. 1st ed. Amsterdam: Elsevier; 1985.

Česla P, Fischer J, Hájek T, Urban J, Holčapek M. In memory of Pavel Jandera. J Sep Sci. 2021;44:3172-3.

Holčapek M, Jandera P, Zderadička P, Hrubá L. Characterization of triacylglycerol and diacylglycerol composition of plant oils using high-performance liquid chromatography-atmospheric pressure chemical ionization mass spectrometry. J Chromatogr A. 2003;1010:195-215.

Holčapek M, Jandera P, Fischer J, Prokeš B. Analytical monitoring of the production of biodiesel by high-performance liquid chromatography with various detection methods. J Chromatogr A. 1999;858:13-31.

Jandera P, Churáček J. Gradient elution in liquid-chromatography. 1. Influence of composition of mobile phase on the capacity ratio (retention volume, band width, and resolution) in isocratic elution - theoretical considerations. J Chromatogr. 1974;91:207-21.

Holčapek M, Lísa M, Jandera P, Kabátová N. Quantitation of triacylglycerols in plant oils using HPLC with APCI-MS, evaporative light-scattering, and UV detection. J Sep Sci. 2005;28:1315-33.

Jandera P. Reversed-phase liquid-chromatography of homologous series - a general method for prediction of retention. J Chromatogr. 1984;314:13-36.

Jandera P, Churáček J. Gradient elution in liquid-chromatography. 2. Retention characteristics (retention volume, band width, resolution, plate number) in solvent-programmed chromatography - theoretical considerations. J Chromatogr. 1974;91:223-35.

Cacciola F, Jandera P, Hajdú Z, Česla P, Mondello L. Comprehensive two-dimensional liquid chromatography with parallel gradients for separation of phenolic and flavone antioxidants. J Chromatogr A. 2007;1149:73-87.

Baczek T, Kaliszan R, Novotná K, Jandera P. Comparative characteristics of HPLC columns based on quantitative structure-retention relationships (QSRR) and hydrophobic-subtraction model. J Chromatogr A. 2005;1075:109-15.

Jandera P, Škeříková V, Řehová L, Hájek T, Baldrianová L, Škopová G, Kellner V, Horna A. RP-HPLC analysis of phenolic compounds and flavonoids in beverages and plant extracts using a CoulArray detector. J Sep Sci. 2005;28:1005-22.

Mondello L, Tranchida PQ, Staněk V, Jandera P, Dugo G, Dugo P. Silver-ion reversed-phase comprehensive two-dimensional liquid chromatography combined with mass spectrometric detection in lipidic food analysis. J Chromatogr A. 2005;1086:91-8.

Jandera P, Churáček J. Ion exchange chromatography of aldehydes, ketones, ethers, alcohols, polyols and saccharides. J Chromatogr. 1974;98:55-104.

Churáček J, Jandera P. Separation of the colored derivatives of some organic compounds using liquid chromatography in small-bore columns packed with ion-exchange resins. J Chromatogr. 1970;53:69-75.

Jandera P, Churáček J, Čáslavský J, Vojáčková M. Liquid chromatography on unmodified and modified spheron gels. I. Nucleosides, bases, and related compounds. Chromatographia 1980;13:734-40.

Jandera P, Churáček J, Szabó D. Liquid chromatography on unmodified and modified spheron gels. II. Barbiturates and sulphonamides. Chromatographia 1981;14:7-12.

Jandera P, Churáček J, Čáslavský J, Szabó D. Liquid chromatography on unmodified and modified spheron gels. III. Phenolic compounds. Chromatographia 1981;14:100-6.

Moravcová D, Jandera P, Urban J, Planeta P. Characterization of polymer monolithic stationary phases for capillary HPLC. J Sep Sci. 2003;26:1005-16.

Moravcová D, Jandera P, Urban J, Planeta P. Comparison of monolithic silica and polymethacrylate capillary columns for LC. J Sep Sci. 2004;27:789-800.

Jandera P, Urban J, Moravcová D. Polymethacrylate and hybrid interparticle monolithic columns for fast separations of proteins by capillary liquid chromatography. J Chromatogr A. 2006;1109:60-73.

Urban J, Moravcová D, Jandera P. A model of flow-through pore formation in methacrylate ester-based monolithic columns. J Sep Sci. 2006;29:1064-73.

Urban J, Škeříková V, Jandera P, Kubíčková R, Pospíšilová M. Preparation and characterization of polymethacrylate monolithic capillary columns with dual hydrophilic interaction reversed-phase mechanism for polar compounds. J Sep Sci. 2009;32:2530-43.

Jandera P, Urban J, Škeříková V, Langmaier P, Kubíčková R, Planeta P. Polymethacrylate monolithic and hybrid particle-monolithic columns for reversed-phase and hydrophilic interaction capillary liquid chromatography. J Chromatogr A. 2010;1217:22-33.

Jandera P, Staňková M, Škeříková V, Urban J. Cross-linker effects on the separation efficiency on (poly)methacrylate capillary monolithic columns. Part I. Reversed-phase liquid chromatography. J Chromatogr A. 2013;1274:97-106.

Staňková M, Jandera P, Škeříková V, Urban J. Cross-linker effects on the separation efficiency on (poly)methacrylate capillary monolithic columns. Part II. Aqueous normal-phase liquid chromatography. J Chromatogr A. 2013;1289:47-57.

Jandera P, Staňková M, Hájek T. New zwitterionic polymethacrylate monolithic columns for one- and two-dimensional microliquid chromatography. J Sep Sci. 2013;36:2430-40.

Jandera P, Hájek T, Staňková M, Vyňuchalová K, Česla P. Optimization of comprehensive two-dimensional gradient chromatography coupling in-line hydrophilic interaction and reversed-phase liquid chromatography. J Chromatogr A. 2012;1268:91-101.

Hájek T, Jandera P, Staňková M, Česla P. Automated dual two-dimensional liquid chromatography approach for fast acquisition of three-dimensional data using combinations of zwitterionic polymethacrylate and silica-based monolithic columns. J Chromatogr A. 2016;1446:91-102.

Staňková M, Jandera P. Dual retention mechanism in two-dimensional LC separations of barbiturates, sulfonamides, nucleic bases and nucleosides on polymethacrylate zwitterionic monolithic micro-columns. Chromatographia 2016;79:657-66.

Jandera P, Hájek T, Šromová Z. Comprehensive two-dimensional monolithic liquid chromatography of polar compounds. J Sep Sci. 2019;42:670-7.

Soczewinski E, Wachtmeister CA. The relation between the composition of certain ternary two-phase solvent systems and RM values. J Chromatogr. 1962;7:311-20.

Snyder LR. Principles of adsorption chromatography. New York: Dekker; 1968.

Jandera P, Kučerová M, Holíková J. Description and prediction of retention in normal-phase high-performance liquid chromatography with Binary and ternary mobile phases. J Chromatogr. 1997;762:15-26.

Snyder LR, Dolan JW, Gant JR. Gradient elution in high-performance liquid chromatography: I. Theoretical basis for reversed-phase systems. J Chromatogr A. 1979;165:3-30.

Vyňuchalová K, Jandera P. Comparison of a C30 bonded silica columns and columns with shorter bonded ligands in reversed-phase LC. Chromatographia 2015;78:861-71.

Bocian S, Soukup J, Matyska M, Pesek J, Jandera P, Buszewski B. The influence of the organic modifier in hydro-organic mobile phase on separation selectivity of steroid hormones separation using cholesterol-bonded stationary phases. J Chromatogr A. 2012;1245:90-7.

Jandera P, Janderová M, Churáček J. Gradient elution in liquid chromatography. 8. Selection of the optimum composition of the mobile phase in liquid chromatography under isocratic conditions. J Chromatogr A. 1978;148:79-97.

Jandera P, Hájek T, Růžičková M. Retention models on core-shell columns. J AOAC Int. 2017;100:1636-46.

Jandera P, Hájek T, Vyňuchalová K. Retention and bandwidths prediction in fast gradient liquid chromatography. Part 2 - Core-shell columns. J Chromatogr A. 2014;1337:57-66.

Jandera P. Reversed-phase liquid-chromatography of homologous series - a general method for prediction of retention. J Chromatogr. 1984;314:13-36.

Jandera P. Methods for characterization of selectivity in reversed-phase liquid chromatography 4. Retention behavior of oligomeric series. J Chromatogr. 1988;449:361-89.

Colin H, Krstulovic AM, Gonnord MF, Guiochon G, Yun Z, Jandera P. Investigation of selectivity in reversed-phase liquid-chromatography - effects of stationary and mobile phases on retention of homologous series. Chromatographia 1983;17:9-15.

Jandera P. A method for characterization and optimization of reversed-phase liquid-chromatographic separations based on the retention behavior in homologous series. Chromatographia 1984;19:101-12.

Jandera P, Rozkosna J. Isocratic and gradient-elution liquid-chromatography of styrene oligomers on silica-gel. J Chromatogr. 1986;362:325-43.

Jandera P. Mechanism and prediction of retention of oligomers in normal-phase and reversed-phase HPLC. Chromatographia 1988;26:417-22.

Jandera P, Urbánek J, Prokeš B, Churáček J. Comparison of various stationary phases for normal-phase high-performance liquid-chromatography of ethoxylated alkylphenols. J Chromatogr. 1990;504:297-318.

Jandera P, Urbánek J. Comparison of chromatographic behavior of oligoethylene glycol nonylphenyl ether nonionic and anionic surfactants in reversed-phase high-performance liquid-chromatography. J Chromatogr A. 1995;689:255-67.

Jandera P, Urbánek J, Prokeš B, Blažková-Brůnová H. Chromatographic behaviour of oligoethylene glycol nonylphenyl ether anionic surfactants in normal-phase high-performance liquid chromatography. J Chromatogr A. 1996;736:131-40.

Jandera P, Holčapek M, Kolářová L. Retention mechanism, isocratic and gradient-elution separation and characterization of (co)polymers in normal-phase and reversed-phase high-performance liquid chromatography. J Chromatogr A. 2000;869:65-84.

Česlová L, Jandera P, Česla P. A study of the thermodynamics of retention of block (co)oligomers using high-performance liquid chromatography/mass spectrometry. J Chromatogr A. 2012;1247:89-98.

Česla P, Vaňková N, Křenková J, Fischer J. Comparison of isocratic retention models for hydrophilic interaction liquid chromatographic separation of native and fluorescently labeled oligosaccharides. J Chromatogr A. 2016;1438:179-88.

Jandera P, Novotná K, Beldean-Galea MS, Jíša K. Retention and selectivity tests of silica-based and metal-oxide bonded stationary phases for RP-HPLC. J Sep Sci. 2006;29:856-71.

Jandera P, Hájek T. Utilization of dual retention mechanism on columns with bonded PEG and diol stationary phases for adjusting the separation selectivity of phenolic and flavone natural antioxidants. J Sep Sci. 2009;32:3603-19.

Jandera P, Hájek T, Škeříková V, Soukup J. Dual hydrophilic interaction-RP retention mechanism on polar columns: structural correlations and implementation for 2-D separations on a single column. J Sep Sci. 2010;33:841-52.

Soukup J, Jandera P. Hydrosilated silica-based columns: the effects of mobile phase and temperature on dual HILIC-RP separation mechanism of phenolic acids. J Chromatogr A. 2012;1228:125-34.

Soukup J, Jandera P. Comparison of nonaqueous hydrophilic interaction chromatography with aqueous normal-phase chromatography on hydrosilated silica-based stationary phases. J Sep Sci. 2013;36:2753-9.

Snyder LR. Principles of gradient elution. Chromatogr Rev. 1965;7:1-51.

Jandera P, Churáček J. Gradient elution in liquid-chromatography. 3. Verification of theoretical relationships for elution characteristics (retention volume, band width and resolution) in isocratic and gradient elution chromatography on silica. J Chromatogr. 1974;93:17-39.

Jandera P, Churáček J. Gradient elution in liquid-chromatography. 4. Verification of theoretical relationships for elution characteristics (retention volume, band width, resolution, plate number) in adsorption chromatography on silica using stepwise and combined (stepwise-gradient) elution and some considerations concerning solvent-programmed and isocratic elution chromatography. J Chromatogr. 1975;104:9-21.

Jandera P, Churáček J. Gradient elution in liquid-chromatography. 5. Cation-exchange chromatography on N,N-dimethyl-para-aminobenzeneazobenzoyl esters and amides in mixed aqueous-organic solutions - influence of nature of exchanger separation. J Chromatogr. 1975;104:23-37.

Jandera P, Churáček J. Gradient elution in liquid-chromatography. 6. Cation-exchange chromatography on N,N-dimethyl-para-aminobenzeneazobenzoyl esters and amides in mixed aqueous-organic solutions - influence of composition of mobile phase on retention characteristics and practical examples of separation. J Chromatogr. 1975;104:257-69.

Jandera P, Janderová M, Churáček J. Gradient elution in liquid-chromatography. 7. Comparison of different mobile phases in adsorption chromatography. J Chromatogr. 1975;115:9-32.

Jandera P, Churáček J. Gradient elution in liquid-chromatography. 9. Selection of optimal conditions in stepwise-elution liquid-chromatography. J Chromatogr. 1979;170:1-10.

Jandera P, Churáček J, Svoboda L. Gradient elution in liquid-chromatography. 10. Retention characteristics in reversed-phase gradient elution chromatography. J Chromatogr. 1979;174:35-50.

Jandera P, Churáček J. Gradient elution in liquid-chromatography. 11. Influence of the adjustable gradient parameters on the chromatographic behavior of sample compounds. J Chromatogr. 1980;192:1-18.

Jandera P, Churáček J. Gradient elution in liquid-chromatography. 12. Optimization of conditions for gradient elution. J Chromatogr. 1980;192:19-36.

Jandera P, Churáček J, Svoboda L. Gradient elution in liquid-chromatography. 13. Instrumental errors in gradient elution chromatography. J Chromatogr. 1980;192:37-51.

Jandera P, Churáček J, Colin H. Gradient elution in liquid-chromatography. 14. Theory of ternary gradients in reversed-phase liquid-chromatography. J Chromatogr. 1981;214:35-46.

Schoenmakers PJ, Billiet HAH, Tijssen R, Degalan L. Gradient selection in reversed-phase liquid-chromatography. J Chromatogr. 1978;149:519-37.

Jandera P, Kučerová M. Prediction of retention in gradient-elution normal-phase high-performance liquid chromatography with binary solvent gradients. J Chromatogr A. 1997;759:13-25.

Jandera P, Hájek T, Šromová Z. Mobile phase effects in reversed-phase and hydrophilic interaction liquid chromatography revisited. J Chromatogr A. 2018;1543:48-57.

Jandera P, Petránek L, Kučerová M. Characterization and prediction of retention in isocratic and gradient-elution normal-phase high-performance liquid chromatography on polar bonded stationary phases with binary and ternary solvent systems. J Chromatogr A. 1997;791:1-19.

Vyňuchalová K, Jandera P. Possibilities of retention prediction in fast gradient liquid chromatography. Part 1 - Comparison of separation on packed fully porous, nonporous and monolithic columns. J Chromatogr A. 2013;1278:37-45.

Jandera P, Hájek T. Possibilities of retention prediction in fast gradient liquid chromatography. Part 3 - Short silica monolithic columns. J Chromatogr A. 2015;1410:76-89.

Jandera P. Predictive calculation methods for optimization of gradient elution using binary and ternary solvent gradients. J Chromatogr. 1989;485:113-41.

Vaňková N, Česla P. Prediction of gradient retention data for hydrophilic interaction liquid chromatographic separation of native and fluorescently labeled oligosaccharides. J Chromatogr A. 2017;1485:82-9.

Vanova J, Malinak D, Andrys R, Kubat M, Mikysek T, Rousarova E, Musilek K, Rousar T, Cesla P. Optimization of gradient reversed-phase high-performance liquid chromatography analysis of acetaminophen oxidation metabolites using linear and non-linear retention model. J Chromatogr A. 2022;1669:462956.

Urban J, Nechvátalová M, Hekerle L. Retention prediction of monoamine neurotransmitters in gradient liquid chromatography. J Sep Sci. Published online Jul 20, 2022. https://doi.org/10.1002/jssc.202200201

Urban J, Jandera P, Kučerová Z, van Straten MA, Claessens HA. A study of the effects of column porosity on gradient separations of proteins. J Chromatogr A. 2007;1167:63-75.

Jandera P, Kučerová Z, Urban J. Retention times and bandwidths in reversed-phase gradient liquid chromatography of peptides and proteins. J Chromatogr A. 2011;1218:8874-89.

Erni F, Frei RW. Two-dimensional column liquid chromatographic technique for resolution of complex mixtures. J Chromatogr A. 1978;149:561-9.

Řehová L, Škeříková V, Jandera P. Optimisation of gradient HPLC analysis of phenolic compounds and flavonoids in beer using a CoulArray detector. J Sep Sci. 2004;27:1345-59.

Jandera P, Novotná K, Kolářová L, Fischer J. Phase system selectivity and peak capacity in liquid column chromatography - the impact on two-dimensional separations. Chromatographia 2004;60:S27-35.

Jandera P, Halama M, Kolářová L, Fischer J, Novotná K. Phase system selectivity and two-dimensional separations in liquid column chromatography. J Chromatogr A. 2005;1087:112-23.

Blahová E, Jandera P, Cacciola F, Mondello L. Two-dimensional and serial column reversed-phase separation of phenolic antioxidants on octadecyl-, polyethyleneglycol-, and pentafluorophenylpropyl-silica columns. J Sep Sci. 2006;29:555-66.

Jandera P, Česla P, Hájek T, Vohralík G, Vyňuchalová K, Fischer J. Optimization of separation in two-dimensional HPLC by adjusting phase system selectivity and using programmed elution techniques. J Chromatogr A. 2008;1189:207-20.

Jandera P, Hájek T, Česla P. Comparison of various second dimension gradient types in comprehensive two-dimensional liquid chromatography. J Sep Sci. 2010;33:1382-97.

Jandera P, Hájek T, Česla P. Effects of the gradient profile, sample volume and solvent on the separation in very fast gradients, with special attention to the second-dimension gradient in comprehensive two-dimensional liquid chromatography. J Chromatogr A. 2011;1218:1995-2006.

Česla P, Hájek T, Jandera P. Optimization of two-dimensional gradient liquid chromatography separations. J Chromatogr A. 2009;1216:3443-57.

Cacciola F, Jandera P, Blahová E, Mondello L. Development of different comprehensive two-dimensional systems for the separation of phenolic antioxidants. J Sep Sci. 2006;29:2500-13.

Hájek T, Škeříková V, Česla P, Vyňuchalová K, Jandera P. Multidimensional LC×LC analysis of phenolic and flavone natural antioxidants with UV-electrochemical coulometric and MS detection. J Sep Sci. 2008;31:3309-28.

Česla P, Křenková J. Fraction transfer process in online comprehensive two-dimensional liquid-phase separations. J Sep Sci. 2016;40:109-23.

Česla P, Váňová J, Hájek T. Three-loop modulation in comprehensive two-dimensional liquid chromatography. In preparation.

Česla P, Fischer J, Jandera P. Separation of phenolic acids and flavone natural antioxidants by two-dimensional method combining liquid chromatography and micellar electrokinetic capillary chromatography. Electrophoresis 2010;31:2200-10.

Česla P, Fischer J, Jandera P. Improvement of the sensitivity of 2D LC-MEKC separation of phenolic acids and flavonoids natural antioxidants using the online preconcentration step. Electrophoresis 2012;33:2464-73.

Holčapek M, Ovčačíková M, Lísa M, Cífková E, Hájek T. Continuous comprehensive two-dimensional liquid chromatography-electrospray ionization mass spectrometry of complex lipidomic samples. Anal Bioanal Chem. 2015;407:5033-43.

Urban J, Jandera P, Schoenmakers P. Preparation of monolithic columns with target mesopore-size distribution for potential use in size-exclusion chromatography. J Chromatogr A. 2007;1150:279-89.

Urban J, Eeltink S, Jandera P, Schoenmakers PJ. Characterization of polymer-based monolithic capillary columns by inverse size-exclusion chromatography and mercury-intrusion porosimetry. J Chromatogr A. 2008;1182:161-8.

Urban J, Jandera P, Langmaier P. Effects of functional monomers on retention behavior of small and large molecules in monolithic capillary columns at isocratic and gradient conditions. J Sep Sci. 2011;34:2054-62.

Jandera P, Holčapek M, Theodoridis G. Investigation of chromatographic behaviour of ethoxylated alcohol surfactants in normal-phase and reversed-phase systems using high-performance liquid chromatography-mass spectrometry. J Chromatogr A. 1998;813:299-311.

Jandera P, Holčapek M, Kolářová L. Retention behavior of oligomers and cooligomers in reversed-phase and in normal-phase interactive liquid chromatographic systems. Int J Polym Anal Charact. 2001;6:261-94.

Wolrab D, Jirasko R, Cifkova E, Horing M, Mei D, Chocholouskova M, Peterka O, Idkowiak J, Hrnciarova T, Kuchar L, Ahrends R, Brumarova R, Friedecky D, Vivo-Truyols G, Skrha P, Skrha J, Kucera R, Melichar B, Liebisch G, Burkhardt R, Wenk MR, Cazenave-Gassiot A, Karasek P, Novotny I, Greplova K, Hrstka R, Holcapek M. Lipidomic profiling of human serum enables detection of pancreatic cancer. Nat Commun. 2022;13:1-16.

Holčapek M, Jandera P, Přikryl J. Analysis of sulphonated dyes and intermediates by electrospray mass spectrometry. Dyes Pigment. 1999;43:127-37.

Holčapek M, Jandera P, Zderadička P. High-performance liquid chromatography-mass spectrometric analysis of sulphonated dyes and intermediates. J Chromatogr A. 2001;926:175-86.

Holčapek M, Volná K, Jandera P, Kolářová L, Lemr K, Exner M, Církva A. Effects of ion-pairing reagents on the electrospray signal suppression of sulphonated dyes and intermediates. J Mass Spectrom. 2004;39:43-50.

Holčapek M, Kolářová L, Růžička A, Jambor R, Jandera P. Structural analysis of ionic organotin(IV) compounds using electrospray tandem mass spectrometry. Anal Chem. 2006;78:4210-8.