The Colorado potato beetle (CPB) is a serious pest of economically important Solanaceae species. The use of essential oil compounds in pest management has been proposed as an alternative to harmful chemical insecticides that disturb human health and ecosystem functioning. We examined the antifeedant activity of three concentrations (0.125%, 0.25% and 0.5%) of pure camphor and a thujone-camphor mixture against 3rd instar larvae and adults. Their efficacy was evaluated according to the degree of leaf damage and avoidance of treated leaves by the CPB. Treatment of potato leaves significantly reduced leaf damage compared to the control. Leaf protection increased at higher concentrations of the examined compounds. Camphor was more effective against larvae and the thujone-camphor mixture was more effective against adults. Additionally, adults moved faster towards the control leaf disc in the two-choice olfactometer assay if an alternative disc was treated with a thujone-camphor mixture, whereas larvae responded similarly to the two potential repellents. However, after contact with the leaf disc treated with the highest compound concentration, the larvae escaped faster from the thujone-camphor mixture than from pure camphor. In conclusion, both examined compounds are promising eco-friendly antifeedants, but their efficacy depends on the developmental stage of the beetle, compound type and applied concentration.

Department of Forest Protection and Wildlife Management Faculty of Forestry and Wood Technology Mendel University Zemědělská 3 61300 Brno Czech Republic

Faculty of Forestry University of Belgrade Kneza Višeslava 1 11030 Belgrade Serbia

Institute for Biological Research Siniša Stanković National Institute of the Republic of Serbia University of Belgrade Bulevar Despota Stefana 142 11060 Belgrade Serbia

Institute for Medicinal Plant Research Dr Josif Pančić Tadeuša Košćuška 1 11000 Belgrade Serbia

Institute for Multidisciplinary Research University of Belgrade Kneza Višeslava 1 11030 Belgrade Serbia

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