Click and Detect: Versatile Ampicillin Aptasensor Enabled by Click Chemistry on a Graphene-Alkyne Derivative
Jazyk angličtina Země Německo Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
Grantová podpora
FW01010183
Technology Agency of the Czech Republic, Program TREND
CZ.02.1.01/0.0/0.0/16_019/0000754
ERDF/ESF project "Nano4Future"
LM2018124
Ministry of Education, Youth and Sports of the Czech Republic
101059266
European Union's Horizon Europe research and innovation program
683024
ERC consolidator grant
19-27454X
Czech Science Foundation
PubMed
36703534
DOI
10.1002/smll.202207216
Knihovny.cz E-zdroje
- Klíčová slova
- antibiotic detection, aptamers, biosensors, graphene acid, screen printed carbon electrodes,
- MeSH
- alkyny MeSH
- ampicilin MeSH
- antibakteriální látky MeSH
- aptamery nukleotidové * chemie MeSH
- biosenzitivní techniky * metody MeSH
- click chemie MeSH
- elektrochemické techniky metody MeSH
- elektrody MeSH
- grafit * chemie MeSH
- limita detekce MeSH
- uhlík chemie MeSH
- zlato chemie MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- alkyny MeSH
- ampicilin MeSH
- antibakteriální látky MeSH
- aptamery nukleotidové * MeSH
- grafit * MeSH
- uhlík MeSH
- zlato MeSH
Tackling the current problem of antimicrobial resistance (AMR) requires fast, inexpensive, and effective methods for controlling and detecting antibiotics in diverse samples at the point of interest. Cost-effective, disposable, point-of-care electrochemical biosensors are a particularly attractive option. However, there is a need for conductive and versatile carbon-based materials and inks that enable effective bioconjugation under mild conditions for the development of robust, sensitive, and selective devices. This work describes a simple and fast methodology to construct an aptasensor based on a novel graphene derivative equipped with alkyne groups prepared via fluorographene chemistry. Using click chemistry, an aptamer is immobilized and used as a successful platform for the selective determination of ampicillin in real samples in the presence of interfering molecules. The electrochemical aptasensor displayed a detection limit of 1.36 nM, high selectivity among other antibiotics, the storage stability of 4 weeks, and is effective in real samples. Additionally, structural and docking simulations of the aptamer shed light on the ampicillin binding mechanism. The versatility of this platform opens up wide possibilities for constructing a new class of aptasensor based on disposable screen-printed carbon electrodes usable in point-of-care devices.
Department of Physical Chemistry Faculty of Science Palacký University Olomouc 771 46 Czech Republic
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