Components of cyanobacterial water blooms were quantified in aerosols above agitated water surfaces of five freshwater bodies. The thoracic and respirable aerosol fraction (0.1-10 µm) was sampled using a high-volume sampler. Cyanotoxins microcystins were detected by LC-MS/MS at levels 0.3-13.5 ng/mL (water) and < 35-415 fg/m3 (aerosol). Lipopolysaccharides (endotoxins) were quantified by Pyrogene rFC assay at levels < 10-119 EU/mL (water) and 0.13-0.64 EU/m3 (aerosol). Cyanobacterial DNA was detected by qPCR at concentrations corresponding to 104-105 cells eq./mL (water) and 101-103 cells eq./m3 (aerosol). Lipopolysaccharides isolated from bloom samples induced IL-6 and IL-8 cytokine release in human bronchial epithelial cells Beas-2B, while extracted cyanobacterial metabolites induced both pro-inflammatory and cytotoxic effects. Bloom components detected in aerosols and their bioactivities observed in upper respiratory airway epithelial cells together indicate that aerosols formed during cyanobacterial water blooms could induce respiratory irritation and inflammatory injuries, and thus present an inhalation health risk.

Department of Aerosol Chemistry and Physics Institute of Chemical Process Fundamentals of the Czech Academy of Sciences Rozvojová 135 16502 Prague Czech Republic

RECETOX Faculty of Science Masaryk University Kotlářská 2 61137 Brno Czech Republic

RECETOX Faculty of Science Masaryk University Kotlářská 2 61137 Brno Czech Republic; Department of Atmospheric Matter Fluxes and Long range Transport Global Change Research Institute of the Czech Academy of Sciences Bělidla 986 4a 60300 Brno Czech Republic

RECETOX Faculty of Science Masaryk University Kotlářská 2 61137 Brno Czech Republic; Department of Experimental Phycology and Ecotoxicology Institute of Botany of the Czech Academy of Sciences Lidická 25 27 60200 Brno Czech Republic

Citace poskytuje Crossref.org

Cyanobacterial Harmful Bloom Lipopolysaccharides Induce Pro-Inflammatory Effects in Immune and Intestinal Epithelial Cells In Vitro