Harvested honey is usually replaced by an alternative sugar to overwinter honeybee colonies. Supplementation of winter stores with beet or cane sucrose is safe for colonies and does not cause winter mortality. Despite this, there are hypotheses that supplementation of inverted sugars has the potential to give better results in overwintering, spring growth, and honey production of the colonies, because bees are consuming already cleaved feed. Therefore, we compared the condition parameters and honey production in 70 colonies at four apiaries overwintered with stores from sucrose or inverted sugars. No statistically significant differences in dependence on the type of the supplemental feed were found. Inverted sugar was more expensive than sucrose for feeding colonies. Economic efficiency, physiological consequences, and other disadvantages of using invert syrups are discussed.

Department of Zoology Fishery Hydrobiology and Apidology Faculty of AgriSciences Mendel University in Brno Zemědělská 1 613 00 Brno Czech Republic

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Johansson T.S.K., Johansson M.P. Feeding sugar to bees. 1. Feeders and syrup feeding. Bee World. 1976;57:137–143. doi: 10.1080/0005772X.1976.11097616. DOI

Furgala B., McCutcheon D.M. Wintering productive colonies. In: Graham J.M., editor. The Hive and the Honeybee. 5th ed. Dadant & Sons; Hamilton, IL, USA: 1992. pp. 829–868.

Ambrose J.T. Management for honey production. In: Graham J.M., editor. The Hive and the Honeybee. 5th ed. Dadant & Sons; Hamilton, IL, USA: 1992. pp. 602–656.

Barker R.J., Lehner Y. Acceptance and sustentative values of honey, the sugars of honey and sucrose fed to caged honey bee workers. Am. Bee J. 1973;113:370–371.

Barker R.J. Considerations in Selecting Sugars for Feeding to Honey Bees. Am. Bee J. 1977;117:76–77.

Bailey L. The effect of acid-hydrolysed sucrose on honeybees. J. Apic. Res. 1966;5:127–136. doi: 10.1080/00218839.1966.11100146. DOI

Doull K.M. Trials with commercial sugar syrups as supplementary or maintenance food for honeybees. Austral. Bee J. 1974;55:17–19.

Barker R.J., Lehner Y. Laboratory comparison of high fructose corn syrup, grape syrup, honey, and sucrose syrup as maintenance food for caged honey bees. Apidologie. 1978;9:111–116. doi: 10.1051/apido:19780203. DOI

Trumpeter J. High Fructose Corn Syrup—An Update. Am. Bee J. 2005;145:893–898.

Severson D.W., Erickson E.H. Honey bee (Hymenoptera: Apidae) colony performance in relation to supplemental carbohydrates. J. Econ. Entomol. 1984;77:1473–1478. doi: 10.1093/jee/77.6.1473. DOI

Sammataro D., Weiss M. Comparison of productivity of colonies of honey bees, Apis mellifera, supplemented with sucrose or high fructose corn syrup. J. Ins. Sci. 2013;13:1–13. doi: 10.1673/031.013.1901. PubMed DOI PMC

Ruiz-Matute A.I., Weiss M., Sammataro D., Finely J., Sanz M.L. Carbohydrate composition of high-fructose corn syrups (HFCS) used for bee feeding: Effect on honey composition. J. Agric. Food Chem. 2010;58:7317–7322. doi: 10.1021/jf100758x. PubMed DOI

Gromisz M. Gospodarka cukrem w pasiekach polskich—Badania ankietowe 1967–1982. Pszczel. Zesz. Nauk. 1985;29:323–338.

Porovnání Krmiva APIVITAL® Sirup a Sacharózy (APIVITAL® Syrup and Sucrose Comparison, in Czech, Partially Also in English, Slovak and Polish Languages) [(accessed on 31 October 2022)]. Available online: https://www.apivital.eu/

Melnichuk I.A. Autumn sugar feeding and physiology of bees (in Russian) Pchelovodstvo. 1964;84:13–15.

Melnichuk I.A. Physiological exhaustion of honeybees after autumn processing of sugar syrup. Trudy nauchno-isledovatelnovo Instituta Pchelovodstva. 1966:79–89. (In Russian)

Jachimowicz T. Wozu brauchen wir Invertzucker bei der Bienenfutterung? Bienenvater. 1976;97:131–133.

Bacílek J., Marek M., Jarý J., Veselý V. Sugar Stores in Honeybee Colonies Fed Sugar Solutions of Different Compositions. J. Apic. Res. 1980;19:187–195. doi: 10.1080/00218839.1980.11100021. DOI

Eijnde van den J., Smeekens S. A comparison between the effects of invert sugars and sucrose on the wintering of honeybee colonies. Maandschrift voor Bijenteelt. 1982;84:243–245. (In Dutch)

Ohe von der W., Schönberger H. Für die Ernährung der Bienen: Futtersirup im Vergleich. Dtsch. Bienen J. 2000;8:312–314.

Ceksteryte V., Racys J. The quality of syrups used for bee feeding before winter and their suitability for bee wintering. J. Apic. Sci. 2006;50:5–14.

Brodschneider R., Moosbeckhofer R., Crailsheim K. Surveys as a tool to record winter losses of honey bee colonies—A 2-year case study in Austria and South Tyrol, Tyrol. J. Apic. Res. 2010;49:23–30. doi: 10.3896/IBRA.1.49.1.04. DOI

Rybak-Chmielewska H., Szczęsna T., Waś E. Attempt to assay maltodextrins occurring in starch syrup and in winter stores made by bees from that syrups. J. Apic. Sci. 2006;50:127–135.

Rybak-Chmielewska H. High performance liquid chromatography (HPLC) study of sugar composition in some kinds of natural honey and winter stores processed by bees from starch syrup. J. Apic. Sci. 2007;51:23–38.

Mirjanic G., Gajger I.T., Mladenovic M., Kozaric Z. Impact of different feed on intestine health of honey bees; Proceedings of the XXXXIII International Apicultural Congress; Kyiv, Ukraine. 29 September–4 October 2013; p. 113.

Crailsheim K., Brodschneider R. Nutrition and health in honey bees. Apidologie. 2010;41:278–294. doi: 10.1051/apido/2010012. DOI

Delaplane K.S., Van Der Stehen J., Guzman-Novoa E. Standard methods for estimating strength parameters of Apis mellifera colonies. J. Apic. Res. 2013;52:1–12. doi: 10.3896/IBRA.1.52.4.12. DOI

Otis G.W., Wheeler D.E., Buck N., Mattila H.R. Storage proteins in winter honey bees. Apiacta. 2004;38:352–357.

Amdam G.V., Omholt S.W. The Regulatory Anatomy of Honeybee Lifespan. J. Theor. Biol. 2002;216:209–228. doi: 10.1006/jtbi.2002.2545. PubMed DOI

Bradford M.M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 1976;72:248–254. doi: 10.1016/0003-2697(76)90527-3. PubMed DOI

Goodwin R.M. Feeding sugar syrup to honey bee colonies to improve pollination: A review. Bee World. 1997;78:56–62. doi: 10.1080/0005772X.1997.11099335. DOI

SAS Institute . SPSSx User’s Guide. McGraw-Hill; New York, NY, USA: 2004. 806p

Free J.B., Spencer-Booth Y. The effect of feeding sugar syrup to honey-bee colonies. J. Agric. Sci. 1961;57:147–151. doi: 10.1017/S0021859600047614. DOI

Barker R.J., Lehner Y. Food choice changes in aging honey bees. Ann. Entomol. Soc. Am. 1974;67:717–718. doi: 10.1093/aesa/67.4.717. DOI

Guler A., Ekinci D., Biyik S., Garipoglu A.V., Onder H., Kocaokutgen H. Effects of feeding honey bees (Hymenoptera: Apidae) with industrial sugars produced by plants using different photosynthetic cycles (carbon C3 and C4) on the colony wintering ability, lifespan, and forage behavior. J. Econ. Entomol. 2018;111:2003–2010. doi: 10.1093/jee/toy189. PubMed DOI

Skubida P. Wpływ zróznicowanego sposobu przygotowania zapasów zimowych na rozwój i produkcyjnosc rodzin. Pszczel. Zesz. Nauk. 1998;42:95–117.

Wegener J., Lorenz M.W., Bienefeld K. Physiological consequences of prolonged nursing in the honey bee. Insectes Soc. 2009;56:85–93. doi: 10.1007/s00040-008-1042-1. DOI

Aschengreen N.H. Production of Glucose-Fructose Syrup. Proc. Biochem. 1975;10:17–19.

Beckedorf S., Rübensaat S., Stampe X., Richardson H. Einfütterung. Dtsch. Bienen, J. 2014;22:14–15.

Szczęsna T., Waś E., Semkiw P., Skubida P., Jaśkiewicz K., Witek M. Changes in the Physicochemical Properties of Starch Syrups after Processing by Honeybees. Agriculture. 2021;11:335. doi: 10.3390/agriculture11040335. DOI

Jachimowicz T., El Sherbiny G. Zur Problematik der Verwendung von Invertzucker für die Bienenfutterung. Apidologie. 1975;6:121–143. doi: 10.1051/apido:19750203. DOI

Monien B.H., Frank H., Seidel A., Glatt H. Conversion of the common food constituent 5-hydroxymethylfurfural into a mutagenic and carcinogenic sulfuric acid ester in the mouse in vivo. Chem. Res. Toxicol. 2009;22:1123–1128. doi: 10.1021/tx9000623. PubMed DOI

Glatt H.R., Sommer Y. Health risks by 5-hydroxymethylfurfural (HMF) and related compounds. In: Skog K., Alexander J., editors. Acrylamide and Other Health Hazardous Compounds in Heat-Treated Foods. 1st ed. Woodhead Publishing; Cambridge, UK: 2006. pp. 328–357.

Zhang X.M., Chan C.C., Stamp D., Minkin S., Archer M.C., Bruce W.R. Initiation and promotion of colonic aberrant crypt foci in rats by 5-hydroxymethy1-2-furaldehyde in thermolyzed sucrose. Carcinogenesis. 1993;14:773–775. doi: 10.1093/carcin/14.4.773. PubMed DOI

LeBlanc B.W., Eggleston G., Sammataro D., Cornett C., Dufault R., Deeby T., St. Cyr E. Formation of Hydroxymethylfurfural in Domestic High-Fructose Corn Syrup and Its Toxicity to the Honey Bee (Apis mellifera) J. Agric. Food Chem. 2009;57:7369–7376. doi: 10.1021/jf9014526. PubMed DOI

Mao W., Schulerb M.A., Berenbaum M.R. Honey constituents up-regulate detoxification and immunity genes in the western honey bee Apis mellifera. Proc. Natl. Acad. Sci. USA. 2013;110:8842–8846. doi: 10.1073/pnas.1303884110. PubMed DOI PMC

Wahl O., Ulm K. Influence of pollen feeding and physiological condition on pesticide sensitivity of the honey bee Apis mellifera carnica. Oecologia. 1983;59:106–128. doi: 10.1007/BF00388082. PubMed DOI

Zábrodská B., Vorlová L. Adulteration of honey and available methods for detection—A review. Acta Vet. Brno. 2014;83:S85–S102. doi: 10.2754/avb201483S10S85. DOI

Özcan M., Arslan D., Ceylan D.A. Effect of inverted saccharose on some properties of honey. Food Chem. 2006;99:24–29. doi: 10.1016/j.foodchem.2005.07.009. DOI

Guler A., Bakan A., Nisbet C., Yavuz O. Determination of important biochemical properties of honey to discriminate pure and adulterated honey with sucrose (Saccharum officinarum L.) syrup. Food Chem. 2007;105:1119–1125. doi: 10.1016/j.foodchem.2007.02.024. DOI

Beckmann K., Beckh G., Lüllmann C. Nachweis von fremder Invertase in Honig. Dtsch. Lebensmitt. Rundsch. 2008;104:55–57.

Voldřich M., Rajchl A., Čížková H., Cuhra P. Detection of Foreign Enzyme Addition into the Adulterated Honey. Czech J. Food Sci. 2009;27:280–282. doi: 10.17221/1066-CJFS. DOI

Fei X., Wu B., Shen C., Zhang R., Ding T., Li L. Determination of exogenous gamma-amylase residue in honey. Chin. J. Chromatogr. Zhongguo Hua Xue Hui. 2012;30:777–781. doi: 10.3724/SP.J.1123.2012.04015. PubMed DOI

Kandolf Borovšak A., Ogrinc N., Lilek N., Korošec M. Feeding honey-bee colonies (Apis mellifera carnica Poll.) and detection of honey adulteration. Acta Aliment. 2016;46:127–136. doi: 10.1556/066.2016.0002. DOI

Pfeiffer K.J., Crailsheim K. Drifting in honeybees. Insectes Soc. 1998;45:151–167. doi: 10.1007/s000400050076. DOI

Hornitzky M.A.Z. The spread of Paenibacillus larvae subsp. larvae infections in an apiary. J. Apic. Res. 1998;37:261–265. doi: 10.1080/00218839.1998.11100981. DOI

Seiler K., Kaufmann A. Kontamination von Honig mit Sulfathiazol durch Raüberei unter Bienen. Mitt. Lebensmitteluntersuchung Hyg. 2002;93:437–446.

Jedrzejczak-Krzepkowska M., Kalinowska H., Bielecki S. β-fructofuranosidase—Properties, structure and applications. Postępy Biochemii. 2011;57:401–410. (In Polish) PubMed