Development of recombinant West Nile virus expressing mCherry reporter protein
Language English Country Netherlands Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
37119976
DOI
10.1016/j.jviromet.2023.114744
PII: S0166-0934(23)00069-1
Knihovny.cz E-resources
- Keywords
- Production of reporter virus, Reporter protein, West Nile virus,
- MeSH
- Humans MeSH
- Mice MeSH
- Recombinant Proteins genetics MeSH
- West Nile virus * genetics MeSH
- West Nile Fever * veterinary MeSH
- Animals MeSH
- Check Tag
- Humans MeSH
- Mice MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Recombinant Proteins MeSH
West Nile virus (WNV) is transmitted to humans and animals by a mosquito and enters the central nervous system, leading to lethal encephalitis. Reporter viruses expressing fluorescent proteins enable detection of infected cells in vitro and in vivo, facilitating evaluation of the dynamics of viral infection, and the development of diagnostic or therapeutic methods. In this study, we developed a method for production of a recombinant replication-competent WNV expressing mCherry fluorescent protein. The expression of mCherry was observed in viral antigen-positive cells in vitro and in vivo, but the growth of the reporter WNV was reduced as compared to the parental WNV. The expression of mCherry was stable during 5 passages in reporter WNV-infected culture cells. Neurological symptoms were observed in mice inoculated intracranially with the reporter WNV. The reporter WNV expressing mCherry will facilitate research into WNV replication in mouse brains.
References provided by Crossref.org
