Physiological responses to honeybee venom poisoning in a model organism, the firebug Pyrrhocoris apterus
Language English Country United States Media print-electronic
Document type Journal Article
PubMed
37196854
DOI
10.1016/j.cbpc.2023.109657
PII: S1532-0456(23)00112-6
Knihovny.cz E-resources
- Keywords
- Adipokinetic hormone, Bee venom, Histamine, Metabolism, Stress,
- MeSH
- Heteroptera * metabolism MeSH
- Histamine pharmacology MeSH
- Insect Hormones * pharmacology MeSH
- Pyrrolidonecarboxylic Acid metabolism MeSH
- Bee Venoms * metabolism MeSH
- Animals MeSH
- Check Tag
- Male MeSH
- Female MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Histamine MeSH
- Insect Hormones * MeSH
- Pyrrolidonecarboxylic Acid MeSH
- Bee Venoms * MeSH
In this study, the biochemical and physiological features of the firebug Pyrrhocoris apterus were investigated to understand the impact of the honeybee Apis mellifera venom on them using physiological methods (mortality, total level of metabolism), biochemical methods (ELISA, mass spectrometry, polyacrylamide gel electrophoresis, spectrophotometry) and molecular methods (real-time PCR). Together, the obtained findings suggest that venom injection increased the level of adipokinetic hormone (AKH) in the CNS of P. apterus, indicating that this hormone plays a key role in activating defence responses. Furthermore, histamine levels in the gut increased significantly after envenomation and did not seem to be modulated by AKH. In contrast, histamine levels in the haemolymph increased after treatment with AKH and AKH + venom. In addition, we found that vitellogenin levels in haemolymph decreased in both males and females after venom application. Lipids, which are the main energy metabolites used by Pyrrhocoris, were significantly exhausted from the haemolymph after the administration of venom and the co-application with AKH reversed this effect. However, we did not find much influence on the effect of digestive enzymes after the injection of venom. Our research has highlighted the noticeable effect of bee venom on P. apterus' body and provided new insights into the role of AKH in controlling defensive responses. However, it is also likely that there will be alternative defence mechanisms.
Faculty of Science Palacký University Olomouc Šlechtitelů 27 783 71 Olomouc Czech Republic
Gymnasium Česká 64 370 21 České Budějovice Czech Republic
Institute of Entomology Biology Centre CAS Branišovská 31 370 05 České Budějovice Czech Republic
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