A neo-functionalized homolog of host transmembrane protein controls localization of bacterial endosymbionts in the trypanosomatid Novymonas esmeraldas
Language English Country England, Great Britain Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
37201521
DOI
10.1016/j.cub.2023.04.060
PII: S0960-9822(23)00542-0
Knihovny.cz E-resources
- Keywords
- Leishmaniinae, Pandoraea, TMEM18, Trypanosomatidae, endosymbiosis,
- MeSH
- Bacteria MeSH
- Eukaryota MeSH
- Symbiosis physiology MeSH
- Trypanosomatina * microbiology MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
The stability of endosymbiotic associations between eukaryotes and bacteria depends on a reliable mechanism ensuring vertical inheritance of the latter. Here, we demonstrate that a host-encoded protein, located at the interface between the endoplasmic reticulum of the trypanosomatid Novymonas esmeraldas and its endosymbiotic bacterium Ca. Pandoraea novymonadis, regulates such a process. This protein, named TMP18e, is a product of duplication and neo-functionalization of the ubiquitous transmembrane protein 18 (TMEM18). Its expression level is increased at the proliferative stage of the host life cycle correlating with the confinement of bacteria to the nuclear vicinity. This is important for the proper segregation of bacteria into the daughter host cells as evidenced from the TMP18e ablation, which disrupts the nucleus-endosymbiont association and leads to greater variability of bacterial cell numbers, including an elevated proportion of aposymbiotic cells. Thus, we conclude that TMP18e is necessary for the reliable vertical inheritance of endosymbionts.
Institute of Microbial Cell Biology Heinrich Heine University 40225 Düsseldorf Germany
Institute of Molecular Medicine Heinrich Heine University 40225 Düsseldorf Germany
Life Science Research Centre Faculty of Science University of Ostrava 710 00 Ostrava Czech Republic
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