Mazamanemorivaga (Cuvier, 1817) is a gray brocket deer that inhabits the Amazon region. An assessment of previous studies revealed inconsistencies in its current taxonomic classification, suggesting the need for an update in its genus classification. A taxonomic repositioning of this species is proposed through the collection of a specimen from its type locality (French Guiana) with subsequent morphological (coloring pattern, body measurements, and craniometry), cytogenetics (G Band, C Band, conventional Giemsa, Ag-NOR staining, and BAC probe mapping), and molecular phylogenetic analysis (mitochondrial genes Cyt B of 920 bp, COI I of 658 bp, D-loop 610 bp), and comparisons with other specimens of the same taxon, as well as other Neotropical deer species. The morphological and cytogenetic differences between this and other Neotropical Cervidae confirm the taxon as a unique and valid species. The phylogenetic analysis evidenced the basal position of the M.nemorivaga specimens within the Blastocerina clade. This shows early diversification and wide divergence from the other species, suggesting that the taxon should be transferred to a different genus. A taxonomic update of the genus name is proposed through the validation of Passalites Gloger, 1841, with Passalitesnemorivagus (Cuvier, 1817) as the type species. Future research should focus on evaluating the potential existence of other species within the genus Passalites, as suggested in the literature.

Central European Institute of Technology Veterinary Research Institute 621 00 Brno Czech Republic Central European Institute of Technology Veterinary Research Institute Brno Czech Republic

Kwata NGO Cayenne French Guiana Kwata NGO Cayenne French Guiana

Laboratorio de Reproducción Animal Departamento de Producción Animal Facultad de Medicina Veterinaria Universidad Nacional Mayor de San Marco San Borja Lima Peru Universidad Nacional Mayor de San Marco Lima Peru

Núcleo de Pesquisa e Conservação de Cervídeos Jaboticabal Brazil

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Abril VV, Duarte JMB. (2008) Chromosome polymorphism in the Brazilian dwarf brocket deer, Mazamanana (Mammalia, Cervidae). Genetics and Molecular Biology 31(1): 53–57. 10.1590/S1415-47572008000100011 DOI

Allen A. (1915) Notes on American deer of the genus Mazama. Bulletin of the American Museum of Natural History 34: 521–553.

Ao D, Yao Y, Li D, Xie M, Ni Q, Zhang M, Xu H. (2020) The complete mitochondrial genome of Siberian roe deer (Capreoluspygargusbedfordi) and its phylogenetic analysis. Mitochondrial DNA B Resource 5(1): 1122–1123. 10.1080/23802359.2019.1711232 PubMed DOI PMC

Artyushin IV, Konorov EA, Kurbakov KA, Stolpovsky YA. (2021) Primer System for Reindeer (Rangifertarandus) Mitochondrial Genome Sequencing. Russian Journal of Genetics 57: 110–11310.1134/S1022795421010038 DOI

Barriga-Bonilla E. (1966) Estudios mastozoológicos colombianos, II. Caldasia 9: 491–502.

Bernegossi AM, Borges CHS, Sandoval EDP, Cernohorska H, Svatava K, Vozdova M, Caparroz R, González S, Duarte JMB. (2022a) Resurrection of the genus Subulo Smith, 1827 for the gray brocket deer, with designation of a neotype. Journal of Mammalogy gyac068. 10.1093/jmammal/gyac068 DOI

Bernegossi AM, Vozdova M, Cernohorska H, Kubickova S, Galindo DJ, Kadlcikova D, Rubes J, Duarte JMB. (2022b) Cytogenetic mapping of cattle BAC probes for the hypothetical ancestral karyotype of the family Cervidae. Cytogenetic and Genome Research 162(3): 140–147. 10.1159/000525592 PubMed DOI

Brooke V. (1878) On the classification of the Cervidae, with a synopsis of the existing species. Proceedings of the Zoological Society of London 1(1): 883–928. 10.1111/j.1469-7998.1878.tb08033.x DOI

Cabrera A. (1960) Catálogo de los mamíferos de América del Sur. Revista Museo Argentino Bernardino Rivadavia 4: 309–732.

Caparroz R, Mantellatto AMB, Bertioli DJ, Figueiredo MG, Duarte JMB. (2015) Characterization of the complete mitochondrial genome and a set of polymorphic microsatellite markers through next-generation sequencing for the brown brocket deer Mazamagouazoubira. Genetics and Molecular Biology 38: 338–345. 10.1590/S1415-475738320140344 PubMed DOI PMC

Carranza J, Roldán M, Peroni EFC, Duarte JMB. (2017) Weak premating isolation between two parapatric brocket deer species. Mammalian Biology 87: 17–26. 10.1016/j.mambio.2017.02.009 DOI

Cifuentes-Rincón A, Morales-Donoso JA, Sandoval EDP, Tomazella IM, Mantellatto AMB, Thoisy B, Duarte JMB. (2020) Designation of a neotype for Mazamaamericana (Artiodactyla, Cervidae) reveals a cryptic new complex of brocket deer species. ZooKeys 958: 143–164. 10.3897/zookeys.958.50300 PubMed DOI PMC

Cuvier GLCFD. (1817) Dictionnarie des Sciences Naturelles. Zoologie. Mammiferes. 7: 1–534.

Czernay S. (1987) Die Spiesshirsche und Pudus: Die Gattungen Mazama und Pudu A. Die Neue Brehm-Bucherei 581: 84.

Dorado-Pérez G. (2012) Prácticas generales de bioquímica y biologia molecular. Departamento de bioquímica y biología molecular. Universidad de Córdoba, España. http://www.uco.es/dptos/bioquimicabiomol/pdfs/47%20SEQUANCIACI%C3%93N%20DNA.pdf

Duarte JMB. (1996) Guia de identificação de cervídeos brasileiros. FUNEP, Jaboticabal, Brazil, 14 pp.

Duarte JMB. (1998) Análise Citogenética e Taxonômica do Gênero Mazama (Cervidae; Artiodactyla) no Brasil. PhD Thesis, Instituto de Biociências, Universidade Estadual Paulista, Botucatu, 1998.

Duarte JMB, Gonzalez S. (2010) Neotropical Cervidology, Biology and Medicine of Latin American Deer. Jaboticabal, Brazil: Funep and Gland, Switzerland, IUCN, 393 pp.

Duarte JMB, Jorge W. (1996) Chromosomal polymorphism in several populations of deer (genus Mazama) from Brazil. Archivos de Zootecnia 45: 281–287.

Duarte JMB, Jorge W. (2003) Morphologic and cytogenetic description of the small red brocket (Mazamabororo Duarte, 1996) in Brazil. Mammalia 67(3): 403–410. 10.1515/mamm.2003.67.3.403 DOI

Duarte JMB, Merino ML. (1997) Taxonomia e Evolução. In: Duarte JMB. (Ed.) Biologia e conservação de Cervideos sul-americanos: Blastocerus, Ozotoceros e Mazama.FUNEP, Jaboticabal, 1–21.

Duarte JMB, Gonzalez S, Maldonado JE. (2008) The surprising evolutionary history of South American Deer. Molecular Phylogenetics and Evolution 49(1): 17–22. 10.1016/j.ympev.2008.07.009 PubMed DOI

Duarte JMB, Boer JA, Sandoval EDP, Bernegossi AM, Tomazella IM. (2021) Skin Freezing Technique for Living Cell Bank. GenProtocols. https://genprotocols.genengnews.com/protocols/skin-freezing-technique-for-living-cell-bank/1041 [Accessed 26 August 2022]

Erxleben JCP. (1777) Systema regni animalis per classes, ordines, genera, species, varietates, cum synonymia et historia Animalium. Classis I. Mammalia. Imprensis Weigandianis, Lipsiae, 1–764. 10.5962/bhl.title.15933 DOI

Fiorillo BF, Sarria-Perea JA, Abril VV, Duarte JMB. (2013) Cytogenetic description of the Amazonian brown brocket Mazamanemorivaga (Artiodactyla; Cervidae). Conparative cytogenetics 7: 25–31. 10.3897/CompCytogen.v7i1.4314 PubMed DOI PMC

Fischer G. (1814) Zoognosia Tabulis Synopticis Illustrata. Typis Nicolai Sergeidis Vsevolozky. Hirsche (Cervi). Sitzungsbericht Konning Akademie Wissenschaften Wien 68: 332–362.

Folmer O, Black M, Hoeh W, Lutz R, Vrijenhoek R. (1994) DNA primers for amplification of mitochondrial Cytochrome C oxidase subunit I from diverse metazoan invertebrates. Molecular Marine Biology and Biotechnology 3: 294–299. PubMed

Frohlich J, Kubickova S, Musilova P, Cernohorska H, Muskova H, Vodicka R, Rubes J. (2017) Karyotype relationships among selected deer species and cattle revealed by bovine FISH probes. PLoS ONE 12(11): 1–17. 10.1371/journal.pone.0187559 PubMed DOI PMC

Galindo DJ, Martins GS, Vozdova M, Cernohorska H, Kubickova S, Bernegossi AM, Kadlcikova D, Rubes J, Duarte JMB. (2021a) Chromosomal polymorphism and speciation: The case of the genus Mazama (Cetartiodactyla; Cervidae). Genes 12(2): 165. 10.3390/genes12020165 PubMed DOI PMC

Galindo DJ, Vozdova M, Kubickova S, Cernohorska H, Bernegossi AM, Kadlcikova D, Rubes J, Duarte JMB. (2021b) Sperm chromosome segregation of rob(4;16) and rob(4;16)inv(4) in the brown brocket deer (Mazamagouazoubira). Theriogenology 168: 33–40. 10.1016/j.theriogenology.2021.03.024 PubMed DOI

Gilbert C, Ropiquet A, Hassanin A. (2006) Mitochondrial and nuclear phylogenies of Cervidae (Mammalia; Ruminantia): Systematics, morphology, and biogeography. Molecular Phylogenetics and Evolution 40(1): 101–117. 10.1016/j.ympev.2006.02.017 PubMed DOI

Gloger CWL. (1841) Gemeinnütziges hand-und Hilfsbuch der Naturgeschichte. Für gebildete Leser aller Stände, besonders für die reifere jugend und ihre Lehrer. U. Schulz und Co. , Breslau [Wroclaw], Poland, 160 pp.

González S, Mantellatto AMB, Duarte JMB. (2018) Craniometrical differentiation of gray brocket deer species from Brazil. Revista del Museo Argentino de Ciencias Naturales 20: 179–193. 10.22179/REVMACN.20.561 DOI

Groves CP, Grubb P. (1987) Relationships of living deer. In: Wemmer CM. (Ed.) Biology and Management of the Cervidae.Smithsonian Institution Press, Washington, 21–59.

Gutiérrez EE, Helgen KM, McDonough MM, Bauer F, Hawkins MTR, Escobedo-Morales LA, Patterson BD, Maldonado JE. (2017) A gene-tree test of the traditional taxonomy of American deer: The importance of voucher specimens, geographic data, and dense sampling. ZooKeys 697: 87–131. 10.3897/zookeys.697.15124 PubMed DOI PMC

Hall TA. (1999) BioEdit: a user-friendly biological sequence alignment editor and analyses program for windows 95/98/NT. In: Nucleic acids symposium series. Information Retrieval Ltd., London, c1979 – c2000, 95–98.

Hammer O, Harper DAT, Ryan PD. (2001) PAST: Paleontological statistics software package for education and data analysis. Palaeontologia Electronica 4: 9.

Hassanin A, Ropiquet A. (2004) Molecular phylogeny of the tribe Bovini (Bovidae, Bovinae) and the taxonomic status of the Kouprey, Bossauveli Urbain 1937. Molecular Phylogenetics and Evolution 3(3): 896–907. 10.1016/j.ympev.2004.08.009 PubMed DOI

Hassanin A, Pasquet E, Vignes JD. (1998) Molecular systematics of the subfamily Caprinae (Artiodactyla, Bovidae) as determined from cytochrome b sequences. Journal of Mammalian Evolution 5(3): 217–236. 10.1023/A:1020560412929 DOI

Hassanin A, Delsuc F, Ropiquet A, Hammer C, Van Vuuren BJ, Matthee C, Ruiz-Garcia M, Catzeflis F, Areskoug V, Nguyen TT, Coulox A. (2012) Pattern and timing of diversification of Cetartiodactyla (Mammalia; Laurasiatheria), as revealed by a comprehensive analysis of mitochondrial genomes. Comptes Rendum Biology 335(1): 32–50. 10.1016/j.crvi.2011.11.002 PubMed DOI

Heckeberg NS, Erpenbeck D, Worheide G, Rossner GE. (2016) Systematic relationship of five newly sequenced cervid species. PeerJ 4: e2307. 10.7717/peerj.2307 PubMed DOI PMC

Heckeberg NS. (2020) The systematics of the Cervidae: A total evidence approach. PeerJ 8: e8114. 10.7717/peerj.8114 PubMed DOI PMC

Hernandez F. (1651) Nova plantarum, animalium et mineralium Mexicanorum Historia. 325 pp. 10.5962/bhl.title.136937 DOI

Hershkovitz P. (1951) Mammals from British Honduras, Mexico, Jamaica and Haiti. Fieldiana. Zoology 31: 547–569. 10.5962/bhl.title.2883 DOI

Hershkovitz P. (1982) Neotropical deer (Cervidae). Part I. Pudus, genus Pudu Gray. Fieldiana-Zoology news series 11: 1–86. 10.5962/bhl.title.5080 DOI

Hollowell T, Reynolds RP. (eds. 2005) Checklist of the Terrestrial Vertebrates of the Guiana Shield. Bulletin of the Biological Society of Washington, no. 13, 1–106. 10.2988/0097-0298(2005)13[1:I]2.0.CO;2 DOI

Howell WW, Black DA. (1980) Controlled silver staining of nucleous organizer regions with a protective colloidal developer: A 1-step method. Experientia 36(8): 1014–1015. 10.1007/BF01953855 PubMed DOI

Illiger JKW. (1815) Uebelblick der Säugethiere nach ihrer Vertheilung über die Welttheile. Abhandlungen der Königlichen Akademie der Wissenschaften zu Berlim 39–159.

Jasper JG, Lee Jr TE, Zabel CJ, Twohy CL, Lane KK, Robertson CS. (2022) Mazamarufina (Artiodactyla: Cervidae). Mammalian Species 54(1016): 212–219. 10.1093/mspecies/seac001 DOI

Kellogg R. (1946) Three new mammals from the Pearl Islands, Panama. Proceedings of the Biological Society of Washington 59: 57–62.

Kerr R. (1792) The animal kingdom or zoological system, of the celebrated Sir Charles Linnaeus. Class I. Mammalia: containing a complete systematic description, arrangement, and nomenclature, of all the known species and varieties of the Mammalia, or animals which give suck to their young; being a translation of that part of the Systema Naturae, as lately published, with great improvements, by Professor Gmelin of Goet tingen.Together with numerous additions from more recent zoological writers, and illustrated with copperplates. A. Strahan,T. Cadell, and W. Creech, Edinburgh, Scotland, xii+1–32+30 (unnumbered) +33–400 pp.

Lesson RP. (1842) Nouveau tableau du règne animal: mammifères (Vol. 1). Arthus Bertrand, Paris, 204 pp.

Lydekker R. (1898) The deer of all lands. Rowland Ward, London, UK, 329 pp.

Medellín R, Gardner A, Aranda M. (1998) The taxonomic status of the Yucatán brown brocket, Mazamapandora (Mammalia: Cervidae). Proceedings of the Biological Society of Washington 111: 1–14.

Merriam CH. (1895) The Earliest Generic Name of an American Deer. Science 1(8): 208–208. 10.1126/science.1.8.208.a PubMed DOI

Miller MA, Pfeiffer W, Schwartz T. (2010) Creating CIPRES Science Gateway for inference of large phylogenetic trees. In: Proceeding of the Gateway Computing Environment Workshop (GCE), 14 nov. 2010, New Orleans, LA, 1–8. 10.1109/GCE.2010.5676129 DOI

Miranda-Ribeiro A. (1914) História natural. Zoologia. Mamíferos. Anexo n°5. Comissão de linhas Telegraficas Estrategicas de Mato Grosso ao Amazonas, Rio de Janeiro.

Oliveira ML, Peres PHF, Gatti A, Morales-Donoso JA, Mangini PR, Duarte JMB. (2020) Faecal DNA and camera traps detect an evolutionarily significant unit of the Amazonian brocket deer in the Brazilian Atlantic Forest. European Journal of Wildlife Research 66(2): 1–10. 10.1007/s10344-020-1367-2 DOI

Ord G. (1815) North American zoology. Guthrie, W. , A New Geographical, Historical and Commercial Grammar: Philadelphia, Johnson and Warner, 2nd American edition, 2, 292 pp.

Osgood WH. (1912) Mammals from western Venezuela and eastern Colombia. Field Museum of Natural History. Zoology (Jena, Germany) 10: 33–68. 10.5962/bhl.title.2647 DOI

Palmer TS. (1904) Index generum mammalium: a list of the genera and families of mammals. North américa Fauna, n. 23, Washington: US Government Printing Office, 1–984. 10.3996/nafa.23.0001 DOI

Pinder L, Leeuwenberg F. (1997) Veado-catingueiro. In: Duarte JMB. (Ed.) Biologia e conservação de Cervídeos sul-americanos: Blastocerus, Ozotoceros e Mazama.Funep, Jaboticabal, 59–68.

Proskuryakova AA, Kulemzina AI, Perelman PL, Makunin AI, Larkin DM, Farré M, Kukekova AV, Johnson JL, Lemskaya NA, Beklemisheva VR, Roelke-Parker ME, Bellizzi J, Ryder AO, O’Brien SJ, Graphodatsky AS. (2017) X Chromosome Evolution in Cetartiodactyla. Genes 8(9): 216. 10.3390/genes8090216 PubMed DOI PMC

Pucheran J. (1851) Note sur une espèce nouvelle de Cerf (Cervusrufinus Bourc. et Puch). Rev et Mag Zool 2(III): 561–565.

Pucheran J. (1852) Monographie Des Especes Du Genre Cerf. Archives du Muséum D`Histoire Naturalle 6: 263–492.

Rafinesque CS. (1817) No title. American Monthly Magazine 1: 363–364.

Rambaut A. (2012) FigTree version 1.4.0. Computer program and documentation. University of Oxford. United Kingdom.

Rees JW. (1969) Morphologic variation in the cranium and mandible of the white-tailed deer (Odocoileusvirginianus): A comparative study of geographical and four biological distances. Journal of Morphology 128(1): 95–112. 10.1002/jmor.1051280105 DOI

Resende JPA. (2012) Comparação cariotípica entre Mazama cinzas do Brasil (Mazamagouazoubira e Mazamanemorivaga, Artiodactyla; Cervidae). Dissertação (Mestrado Melhoramento Genético Animal) Universidade Estadual Paulista. Faculdade de Ciências Agrarias e Veterinárias. Jaboticabal, 153 pp.

Rossi RV. (2000) Taxonomia de Mazama Rafinesque, 1817 do Brasil (Artiodactyla, Cervidae). Dissertação (Mestrado em Ciencias Biologicas) Instituto de Biociências. Universidade de São Paulo, São Paulo, 147 pp.

Rossi RV, Duarte JMB. (2008) Mazamanemorivaga. in: IUCN 2008. IUCN Red List of Threatened Species. www.iucnredlist.org

Rossi RV, Bodmer R, Duarte JMB, Trovati RG. (2010) Amazonian Brown Brocket Deer. Mazamanemorivaga (Cuvier, 1817). In: Duarte JMB, González S. (Eds) Neotropical Cervidology, Biology and Medicine of Latin American Deer, Jaboticabal, Brazil.FUNEP/IUCN, Jaboticabal/Gland, 202–210.

Sambrook J, Fritsch E, Maniatis T. (1989) Molecular cloning: A Laboratory Manual. Cold Spring Harbor Press, New York.

Saussure (1860) Note sur quelques mammifères du Mexique. Revue et Magasin de Zoologie, Paris Series 2: 122–152.

Seabright MA. (1971) A rapid banding technique for human chromosomes. Lancet 1(7731): 9971–9972. 10.1016/S0140-6736(71)90287-X PubMed DOI

Smith HC. (1827) The seventh order of the Mammalia. The Ruminantia. In: Griffith E, Smith CH, Pidgeon E. (Eds) The animal kingdom arranged in conformity with its organization, by the Baron Cuvier, with additional descriptions of all the species hitherto named, and many not before noticed, by Edward Griffith and others.Vol. 5. Geo B. Whitaker, London, 298–376.

Sumner AT. (1972) A simple technique for demonstrating centromeric heterochromatin. Experimental Cell Research 75(1): 304–306. 10.1016/0014-4827(72)90558-7 PubMed DOI

Świsłocka M, Borkowska A, Matosiuk M, Czajkowska M, Duda N, Kowalczyk R, Ratkiewicz M. (2020) A Sex-biased polyparasitism in moose (Alcesalces) based on molecular analysis of faecal samples. International Journal for Parasitology: Parasites and Wildlife 13: 171–177. 10.1016/j.ijppaw.2020.10.008 PubMed DOI PMC

Tate G. (1939) The mammals of the Guiana region. Bulletin of the American Museum of Natural History 76: 151–229.

Varela DM, Trovati RG, Guzman KR, Rossi RV, Duarte JMB. (2010) Red brocket deer Mazamaamericana (Erxleben, 1777). In: Duarte JMB, González S. (Eds) Neotropical Cervidology, Biology and Medicine of Latin American Deer.FUNEP/IUCN, Jaboticabal/Gland, 151–159.

Verma RS, Babu A. (1995) Human chromosomes: principles and techniques. McGraw Hill, New York, 2nd edn., 419 pp.

Vilá C, Amorim I, Leonard JA, Posada D, Castroviejo J, Petricci-Fonseca F, Crandall KA, Wayne RK. (1999) Mitochondrial DNA phytogeography and population history of the grey wolf Canislupus. Molecular Ecology 8(12): 2089–2103. 10.1046/j.1365-294x.1999.00825.x PubMed DOI

von den Driesch A. (1976) A guide to the measurement of animal bones from archaeological sites. Peabody Museum Bulletin 1, Harvard University, Peabody Museum of Archaeology and Ethnology, 148 pp.

Vozdova M, Kubickova S, Cernohorska H, Fröhlich J, Vodicka R, Rubes J. (2019) Comparative Study of the Bush Dog (Speothosvenaticus) Karyotype and analysis of satellite DNA sequences and their chromosome distribution in six species of Canidae. Cytogenetic and Genome Research 159(2): 88–96. 10.1159/000503082 PubMed DOI

Wagner JA. (1855) Die Saugthiere in Abbildungen nach der natur, mit beschreibungen/ von D. Johan Cristian Daniel von Schreber; fort gesetzt von D. August Goldfuss Johann Andreas Wagner. Erlangen, W. Walter. Supplementband.