Açaí, Euterpe oleracea Mart., is a native plant from the Amazonian and is rich in several phytochemicals with anti-tumor activities. The aim was to analyze the effects of açaí seed oil on colorectal adenocarcinoma (ADC) cells. In vitro analyses were performed on CACO-2, HCT-116, and HT-29 cell lines. The strains were treated with açaí seed oil for 24, 48, and 72 h, and cell viability, death, and morphology were analyzed. Molecular docking was performed to evaluate the interaction between the major compounds in açaí seed oil and Annexin A2. The viability assay showed the cytotoxic effect of the oil in colorectal adenocarcinoma cells. Acai seed oil induced increased apoptosis in CACO-2 and HCT-116 cells and interfered with the cell cycle. Western blotting showed an increased expression of LC3-B, suggestive of autophagy, and Annexin A2, an apoptosis regulatory protein. Molecular docking confirmed the interaction of major fatty acids with Annexin A2, suggesting a role of açaí seed oil in modulating Annexin A2 expression in these cancer cell lines. Our results suggest the anti-tumor potential of açaí seed oil in colorectal adenocarcinoma cells and contribute to the development of an active drug from a known natural product.

Cell Structure and Dynamics Group Cellular and Molecular Oncobiology Program National Cancer Institute Rio de Janeiro 20231 050 Brazil

Department of Chemistry Faculty of Science University of Hradec Kralove Rokitansheho 62 500 03 Kralove Czechia

Faculty of Medical Science Post graduation in Internal Medicine State University of Campinas Campinas 13083 970 Brazil

Faculty of Pharmaceutical Sciences Post graduation in Internal Medicine State University of Campinas Campinas 13083 970 Brazil

Laboratory of Molecular Modeling Applied to Chemical and Biological Defense Military Institute of Engineering Rio de Janeiro 22290 270 Brazil

Nucleum of Basic and Applied Immunology Pathology Department Federal University of Maranhão São Luís 65080 805 Brazil

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