Modulation of the capillary leakage by exogenous albumin in a rat model of endothelial glycocalyx damage
Language English Country United States Media print
Document type Journal Article
PubMed
38073383
DOI
10.3233/ch-232027
PII: CH232027
Knihovny.cz E-resources
- Keywords
- Albumin, endothelial glycocalyx, evans blue, microcirculation, spectrophotometry,
- MeSH
- Albumins * metabolism MeSH
- Endothelium, Vascular drug effects metabolism MeSH
- Evans Blue MeSH
- Glycocalyx * metabolism drug effects MeSH
- Hyaluronoglucosaminidase pharmacology MeSH
- Capillary Permeability * drug effects MeSH
- Rats MeSH
- Humans MeSH
- Disease Models, Animal MeSH
- Rats, Wistar MeSH
- Animals MeSH
- Check Tag
- Rats MeSH
- Humans MeSH
- Male MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Albumins * MeSH
- Evans Blue MeSH
- Hyaluronoglucosaminidase MeSH
BACKGROUND: Endothelial glycocalyx (EG) plays a crucial role in maintaining the plasma proteins within the intravascular space. OBJECTIVE: We studied whether exogenous albumin protects the EG in an experimental model of EG enzymatic damage in rats. METHODS: Rats were divided into three groups of 10 animals that received (1) Evans blue (2) Evans blue + hyaluronidase, or (3) Evans blue + hyaluronidase + 20% human albumin via the tail vein. Spectrophotometric analysis was performed 2 h later to quantify the leakage of Evans blue-labeled albumin into the heart, lungs, brain, kidneys, liver, small intestine, spleen, and skeletal muscle. RESULTS: Administration of hyaluronidase numerically increased the capillary leakage of Evans blue in all examined tissues. Co-administration of albumin decreased the leakage of albumin in all tissues except the heart. In the lungs, the ratio between the absorbance and dry organ weight decreased from 5.3 ± 2.4 to 1.7 ± 0.5 (mean ± SD) (P < 0.002), and in the liver, the absorbance decreased from 2.2 ± 0.7 to 1.5 ± 0.4 (P < 0.011). CONCLUSION: Exogenous albumin decreased the capillary leakage of albumin which was interpreted as a sign of maintained EG integrity.
1st Department of Anaesthesiology and Intensive Therapy Medical University of Lublin Lublin Poland
Department of Microbiology and Immunology Dalhousie University Halifax NS Canada
Department of Pharmacology Dalhousie University Halifax NS Canada
Department of Physiology and Biophysics Dalhousie University Halifax NS Canada
Faculty of Health Studies Technical University in Liberec Liberec Czech Republic
Faculty of Medicine in Hradec Kralove Charles University Prague Czech Republic
Faculty of Military Health Sciences University of Defence Hradec Kralove Czech Republic
International Fluid Academy Lovenjoel Belgium
Karolinska Institutet at Danderyds Hospital Stockholm Sweden
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