We report a novel impedimetric sensor based on a graphite electrode impregnated with polyethylene and paraffin under vacuum (IGE) modified with electrochemically deposited gold and a self-assembled monolayer of N-acetyl-L-cysteine (NAC/Au/IGE) for selective and sensitive determination of extracellular hydroxyl radicals (OH•) generated by living cells. The application of a sulphur-containing molecule oxidized by OH• predicts the high selectivity of the sensor, and the utilization of the non-faradaic impedance spectroscopy for recording an analytical response makes it possible to achieve superior sensitivity with a detection limit of 0.01 nM and a linear dynamic range of 0.08-8 nM. Meanwhile, NAC/Au/IGE demonstrated a strong potential of detecting OH• generated by biological objects via successful determination of extracellular hydroxyl radicals generated by normal fibroblast cells and prostate carcinoma cells.

Charles University Faculty of Science Department of Analytical Chemistry UNESCO Laboratory of Environmental Electrochemistry Hlavova 8 2030 CZ 128 43 Prague 2 Czech Republic

Division for Chemical Engineering School of Earth Sciences and Engineering National Research Tomsk Polytechnic University Lenin Avenue 30 634050 Tomsk Russia

Institute of Strength Physics and Materials Science of Siberian Branch of Russian Academy of Sciences Pr Akademicheskii 2 4 634055 Tomsk Russia

Research School of Chemistry and Applied Biomedical Sciences National Research Tomsk Polytechnic University 634050 Tomsk Russia

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