A novel impedimetric sensor based on N-acetyl-L-cysteine for the determination of hydroxyl radicals in cell cultures in vitro
Language English Country Netherlands Media print-electronic
Document type Journal Article
PubMed
38159349
DOI
10.1016/j.talanta.2023.125600
PII: S0039-9140(23)01351-6
Knihovny.cz E-resources
- Keywords
- Cell cultures, Hydroxyl radical, Impedimetric sensor, N-Acetyl-L-cysteine, Oxidative stress,
- MeSH
- Acetylcysteine MeSH
- Biosensing Techniques * methods MeSH
- Cell Culture Techniques MeSH
- Electrochemical Techniques methods MeSH
- Electrodes MeSH
- Hydroxyl Radical * MeSH
- Immunoglobulin E MeSH
- Limit of Detection MeSH
- Gold chemistry MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Acetylcysteine MeSH
- Hydroxyl Radical * MeSH
- Immunoglobulin E MeSH
- Gold MeSH
We report a novel impedimetric sensor based on a graphite electrode impregnated with polyethylene and paraffin under vacuum (IGE) modified with electrochemically deposited gold and a self-assembled monolayer of N-acetyl-L-cysteine (NAC/Au/IGE) for selective and sensitive determination of extracellular hydroxyl radicals (OH•) generated by living cells. The application of a sulphur-containing molecule oxidized by OH• predicts the high selectivity of the sensor, and the utilization of the non-faradaic impedance spectroscopy for recording an analytical response makes it possible to achieve superior sensitivity with a detection limit of 0.01 nM and a linear dynamic range of 0.08-8 nM. Meanwhile, NAC/Au/IGE demonstrated a strong potential of detecting OH• generated by biological objects via successful determination of extracellular hydroxyl radicals generated by normal fibroblast cells and prostate carcinoma cells.
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