Organoids are 3D cell cultures with microanatomies mimicking aspects of real organs, useful for e.g. animal-free studies of development, disease, and drug discovery. The cell medium of organoid models of Langerhans islets, regulating blood glucose levels by insulin secretion, can be analyzed by liquid chromatography-mass spectrometry (LC-MS). However, organoid medium complexity is a major challenge, as matrix interferences can reduce sensitivity and selectivity, even with optimized LC-MS conditions. By applying preparative agarose gel electrophoresis-electrodialysis (PGE-ED), we were able to decrease the cell medium background signal, allowing for reduced interferences affecting LC-MS analysis of human insulin.

Czech Academy of Sciences Institute of Analytical Chemistry Veveří 967 97 Brno 602 00 Czech Republic

Czech Academy of Sciences Institute of Analytical Chemistry Veveří 967 97 Brno 602 00 Czech Republic; Department of Chemistry Faculty of Science Masaryk University Kamenice 5 Brno 625 00 Czech Republic

Department of Chemistry University of Oslo Blindern Oslo Norway; Hybrid Technology Hub Centre of Excellence Institute of Basic Medical Sciences Faculty of Medicine University of Oslo Oslo Norway

Hybrid Technology Hub Centre of Excellence Institute of Basic Medical Sciences Faculty of Medicine University of Oslo Oslo Norway

Hybrid Technology Hub Centre of Excellence Institute of Basic Medical Sciences Faculty of Medicine University of Oslo Oslo Norway; Department of Transplant Medicine and Institute for Surgical Research Oslo University Hospital Oslo Norway

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An FDA-Validated, Self-Cleaning Liquid Chromatography-Mass Spectrometry System for Determining Small-Molecule Drugs and Metabolites in Organoid/Organ-on-Chip Medium