Super-resolution techniques expand the abilities of researchers who have the knowledge and resources to either build or purchase a system. This excludes the part of the research community without these capabilities. Here we introduce the openSIM add-on to upgrade existing optical microscopes to Structured Illumination super-resolution Microscopes (SIM). The openSIM is an open-hardware system, designed and documented to be easily duplicated by other laboratories, making super-resolution modality accessible to facilitate innovative research. The add-on approach gives a performance improvement for pre-existing lab equipment without the need to build a completely new system.

BioFrontiers Center University of Colorado Colorado Springs Colorado Springs CO USA

Center for Innovation in Global Health Stanford University Stanford CA USA

Department of Medical Biology UiT The Arctic University of Norway Tromsø Norway

Faculty of Electrical Engineering Czech Technical University Prague Prague Czech Republic

School of Engineering Swiss Federal Institute of Technology Lausanne Switzerland

School of Life Sciences Swiss Federal Institute of Technology Lausanne Switzerland

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Open-source microscope add-on for structured illumination microscopy