Organotypic 3D Cell Culture of the Embryonic Lacrimal Gland
Language English Country United States Media print
Document type Journal Article
- Keywords
- 3D culture, Branching, Development, Epithelium, Lacrimal gland, Morphogenesis,
- MeSH
- Epithelium MeSH
- Morphogenesis MeSH
- Mice MeSH
- Organ Culture Techniques MeSH
- Lacrimal Apparatus * MeSH
- Cell Culture Techniques, Three Dimensional MeSH
- Animals MeSH
- Check Tag
- Mice MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
Ectodermal organ development, including lacrimal gland, is characterized by an interaction between an epithelium and a mesenchyme. Murine lacrimal gland is a good model to study non-stereotypical branching morphogenesis. In vitro cultures allow the study of morphogenesis events with easy access to high-resolution imaging. Particularly, embryonic lacrimal gland organotypic 3D cell cultures enable the follow-up of branching morphogenesis thanks to the analysis of territories organization by immunohistochemistry. In this chapter, we describe a method to culture primary epithelial fragments together with primary mesenchymal cells, isolated from embryonic day 17 lacrimal glands.
Department of Histology and Embryology Faculty of Medicine Masaryk University Brno Czech Republic
Université Paris Cité CNRS Institut Jacques Monod Paris France
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Dhouailly D (2009) A new scenario for the evolutionary origin of hair, feather, and avian scales. J Anat 214:587–606. https://doi.org/10.1111/j.1469-7580.2008.01041.x PubMed DOI PMC
Makarenkova HP, Ito M, Govindarajan V et al (2000) FGF10 is an inducer and Pax6 a competence factor for lacrimal gland development. Development 127:2563–2572. https://doi.org/10.1242/dev.127.12.2563 PubMed DOI
Kuony A, Michon F (2017) Epithelial markers aSMA, Krt14, and Krt19 unveil elements of murine lacrimal gland morphogenesis and maturation. Front Physiol 8. https://doi.org/10.3389/fphys.2017.00739
Kuony A, Ikkala K, Kalha S et al (2019) Ectodysplasin-a signaling is a key integrator in the lacrimal gland–cornea feedback loop. Development 146. https://doi.org/10.1242/dev.176693
Munne PM, Närhi K, Michon F (2013) Analysis of tissue interactions in ectodermal organ culture. Methods Mol Biol 945:401–416. https://doi.org/10.1007/978-1-62703-125-7_24 PubMed DOI
Bannier-Hélaouët M, Post Y, Korving J et al (2021) Exploring the human lacrimal gland using organoids and single-cell sequencing. Cell Stem Cell 28:1221–1232.e7. https://doi.org/10.1016/j.stem.2021.02.024 PubMed DOI
Hirayama M, Tsubota K, Tsuji T (2017) Generation of a bioengineered lacrimal gland by using the organ germ method. In: Tsuji T (ed) Organ regeneration: 3D stem cell culture & manipulation. Springer New York, New York, pp 153–165 DOI
Sumbal J, Chiche A, Charifou E et al (2020) Primary mammary organoid model of lactation and involution. Front Cell Dev Biol 8:68. https://doi.org/10.3389/fcell.2020.00068 PubMed DOI PMC
Finley JK, Farmer D, Emmerson E et al (2014) Manipulating the murine lacrimal gland. J Vis Exp:e51970. https://doi.org/10.3791/51970
Sumbal J, Koledova Z (2022) Single organoids droplet-based staining method for high-end 3D imaging of mammary organoids. In: Vivanco M, dM. (eds) Mammary stem cells: methods and protocols. Springer US, New York, pp 259–269. https://doi.org/10.1007/978-1-0716-2193-6_14
