Ectodermal organ development, including lacrimal gland, is characterized by an interaction between an epithelium and a mesenchyme. Murine lacrimal gland is a good model to study non-stereotypical branching morphogenesis. In vitro cultures allow the study of morphogenesis events with easy access to high-resolution imaging. Particularly, embryonic lacrimal gland organotypic 3D cell cultures enable the follow-up of branching morphogenesis thanks to the analysis of territories organization by immunohistochemistry. In this chapter, we describe a method to culture primary epithelial fragments together with primary mesenchymal cells, isolated from embryonic day 17 lacrimal glands.

Department of Histology and Embryology Faculty of Medicine Masaryk University Brno Czech Republic

Laboratory of Tissue Morphogenesis and Cancer Institute of Molecular Genetics of the Czech Academy of Sciences Prague Czech Republic

Université Paris Cité CNRS Institut Jacques Monod Paris France

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