Early-Onset Neonatal Sepsis: Inflammatory Biomarkers and MicroRNA as Potential Diagnostic Tools in Preterm Newborns
Jazyk angličtina Země Česko Médium print
Typ dokumentu časopisecké články
Grantová podpora
NU20-07-00109
Agentura Pro Zdravotnický Výzkum České Republiky
PubMed
38583178
DOI
10.14712/fb2023069050173
PII: fb_2023069050173
Knihovny.cz E-zdroje
- Klíčová slova
- endothelial biomarkers, inflammation, inflammatory markers, microRNA, newborn, sepsis,
- MeSH
- biologické markery metabolismus MeSH
- interleukin-18 MeSH
- kojenec MeSH
- lidé MeSH
- mikro RNA * genetika MeSH
- novorozenec MeSH
- novorozenecká sepse * diagnóza MeSH
- sepse * diagnóza genetika MeSH
- vaskulární endoteliální růstový faktor A MeSH
- zánět MeSH
- Check Tag
- kojenec MeSH
- lidé MeSH
- novorozenec MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- biologické markery MeSH
- interleukin-18 MeSH
- mikro RNA * MeSH
- vaskulární endoteliální růstový faktor A MeSH
Mortality and morbidity of newborns with sepsis can be improved by early and accurate diagnosis and targeted therapy. To evaluate the early molecular events associated with inflammation and infection, we evaluated markers of endothelial activation and injury and circulating plasma miRNAs in preterm newborns with sepsis. The study group consisted of newborns with gestational age ≤ 32 weeks, with culture-positive early-onset neonatal sepsis (sepsis group, N = 8), and as a control group, we enrolled newborns without sepsis (control group, N = 12). Soluble markers of inflammation were measured using Luminex-based multiplex assay. Platelet-free plasma RNA was used to construct the library for miRNA sequencing analysis. Normalized counts were calculated and used to measure differential expression of individual detected miRNAs. We found a significant increase of interleukin 18 (IL-18) in the cord blood of the sepsis group (mean ± SEM, 104.7 ± 30.4 pg/ml vs 52.7 ± 5.6 pg/ml, P = 0.02). In peripheral blood of sepsis group patients, we found a significant increase of VEGF-A compared to controls (196.0 ± 70.5 pg/ml vs 59.6 ± 8.5 pg/ml, P = 0.02). In the cord blood plasma, eight miRNAs had significantly differential expression (P < 0.05), four miRNAs were up-regulated and four miRNAs down-regulated. In peripheral blood plasma, all nine miRNAs with significant differential expression were up-regulated. In conclusion, in early-onset neonatal sepsis, IL-18 and VEGF-A might be considered in diagnostic workup. Early-onset sepsis in preterm newborns is associated with significant changes in the circulating miRNA pattern.
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