Svornostia abyssi gen. nov., sp. nov. isolated from the world's deepest silver-uranium mine currently devoted to the extraction of radon-saturated water
Jazyk angličtina Země Anglie, Velká Británie Médium print
Typ dokumentu časopisecké články
PubMed
38922323
DOI
10.1099/ijsem.0.006432
Knihovny.cz E-zdroje
- Klíčová slova
- Svornostia abyssi, mineral springs, novel bacterial taxa, slow-growing bacterium,
- MeSH
- DNA bakterií * genetika MeSH
- fylogeneze * MeSH
- hornictví * MeSH
- kyselina diaminopimelová analýza MeSH
- mastné kyseliny * chemie analýza MeSH
- mikrobiologie vody MeSH
- peptidoglykan MeSH
- RNA ribozomální 16S * genetika MeSH
- sekvenční analýza DNA * MeSH
- stříbro MeSH
- techniky typizace bakterií * MeSH
- vitamin K 2 analogy a deriváty analýza MeSH
- zastoupení bazí * MeSH
- Publikační typ
- časopisecké články MeSH
- Geografické názvy
- Česká republika MeSH
- Názvy látek
- DNA bakterií * MeSH
- kyselina diaminopimelová MeSH
- mastné kyseliny * MeSH
- menaquinone 7 MeSH Prohlížeč
- peptidoglykan MeSH
- RNA ribozomální 16S * MeSH
- stříbro MeSH
- vitamin K 2 MeSH
A Gram-stain-positive, rod-shaped, aerobic, motile bacterium, J379T, was isolated from radioactive water spring C1, located in a former silver-uranium mine in the Czech Republic. This slow-growing strain exhibited optimal growth at 24-28 °C on solid media with <1 % salt concentration and alkaline pH 8-10. The only respiratory quinone found in strain J379T was MK-7(H4). C18 : 1 ω9c (60.9 %), C18 : 0 (9.4 %), C16 : 0 and alcohol-C18 : 0 (both 6.2 %) were found to be the major fatty acids. The peptidoglycan contained directly cross-linked meso-diaminopimelic acid. Phylogenetic reconstruction based on the 16S rRNA gene sequences and the core-genome analysis revealed that strain J379T forms a separate phylogenetic lineage within the recently amended order Solirubrobacterales. A comparison of the 16S rRNA gene sequences between strain J379T and other members of the order Solirubrobacterales showed <96 % similarity. This analysis revealed that the closest type strains were Parviterribacter kavangonensis D16/0 /H6T (95.2 %), Capillimicrobium parvum 0166_1T (94.9 %) and Conexibacter arvalis KV-962T (94.5 %). Whole-genome analysis showed that the closest type strain was Baekduia soli BR7-21T with an average nucleotide identity of 78 %, average amino acid identity of 63.2 % and percentage of conserved proteins of 48.2 %. The G+C content of the J379T genomic DNA was 71.7 mol%. Based on the phylogenetic and phylogenomic data, as well as its physiological characteristics, strain J379T is proposed to represent a type strain (DSM 113746T=CCM 9300T) of Svornostia abyssi gen. nov. sp. nov. within the family Baekduiaceae.
Institute of Microbiology of the Czech Academy of Sciences Prague Czech Republic
Leibniz institute DSMZ German Collection of Microorganisms and Cell Cultures Braunschweig Germany
Military Health Institute Ministry of Defence of the Czech Republic Prague Czech Republic
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