Oxidative stress status, as a disruption of redox homeostasis, in the blood sera of Wistar rats caused by repeated application of selected acetylcholinesterase reactivators - asoxime, obidoxime, K027, K048, K074, and K075 were evaluated. Throughout this study, each oxime in a dose of 0.1 of LD50/kg im was given 2x/week for 4 weeks. Then, seven days after the last oximes' application, markers of lipid peroxidation (malondialdehyde, MDA), and protein oxidation (advanced oxidation protein products, AOPP), as well as the activity of antioxidant enzymes (catalase, CAT, superoxide dismutase, SOD, reduced glutathione, GSH, and oxidized glutathione, GSSG), were determined. Oxidative stress parameters, MDA and AOPP were significantly highest in the K048-, K074- and K075-treated groups (p < 0.001). The activity of CAT was significantly elevated in the obidoxime-treated group (p < 0.05), while treatment with K027, K048, and K074 induced high elevation in SOD levels (p < 0.01, p < 0.001). Interestingly, the activity of GSH in each oxime-treated group was significantly elevated. Unlike, treatment with obidoxime caused elevation in GSSG levels (p < 0.01). As a continuation of our previously published data, these results assure that applied oximes following subacute treatment ameliorated the oxidative status and further adverse systemic toxic effects in rats.

Centre for Biomedical Research Faculty of Medicine University of Banja Luka Save Mrkalja 14 78000 Banja Luka Bosnia and Herzegovina; Department of Human Genetics Faculty of Medicine University of Banja Luka Save Mrkalja 14 78000 Banja Luka Bosnia and Herzegovina

Department for Experimental Toxicology and Pharmacology National Poison Control Centre Military Medical Academy Crnotravska 17 11040 Belgrade Serbia; Medical Faculty of the Military Medical Academy University of Defence Crnotravska 17 11040 Belgrade Serbia; Department of Chemistry Faculty of Science University of Hradec Kralove Rokitanskeho 62 500 03 Hradec Kralove Czech Republic

Department of Chemistry Faculty of Science University of Hradec Kralove Rokitanskeho 62 500 03 Hradec Kralove Czech Republic

Department of Chemistry Faculty of Science University of Hradec Kralove Rokitanskeho 62 500 03 Hradec Kralove Czech Republic; Biomedical Research Center University Hospital Hradec Kralove 50005 Hradec Kralove Czech Republic

Department of Human Genetics Faculty of Medicine University of Banja Luka Save Mrkalja 14 78000 Banja Luka Bosnia and Herzegovina

Department of Pharmacology Toxicology and Clinical Pharmacology Faculty of Medicine University of Banja Luka Save Mrkalja 14 78000 Banja Luka Bosnia and Herzegovina

Special Police Unit Ministry of Interior Trebevićka 12 A 11 030 Belgrade Serbia

University of Belgrade Faculty of Pharmacy Department of Pharmaceutical Chemistry Vojvode Stepe 450 11121 Belgrade Serbia

University of Belgrade Institute for Medical Research National Institute of the Republic of Serbia Department for Cardiovascular Research Dr Subotića 4 11 132 Belgrade Serbia

Veterinary Services Center Military Health Department Crnotravska 17 11040 Belgrade Serbia

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