Juvenile hormone (JH) signaling is realized at the gene regulatory level by receptors of the bHLH-PAS transcription factor family. The sesquiterpenoid hormones and their synthetic mimics are agonist ligands of a unique JH receptor (JHR) protein, methoprene-tolerant (MET). Upon binding an agonist to its PAS-B cavity, MET dissociates from a cytoplasmic chaperone complex including HSP83 and concomitantly switches to a bHLH-PAS partner taiman, forming a nuclear, transcriptionally active JHR heterodimer. This course of events resembles the vertebrate aryl hydrocarbon receptor (AHR), activated by a plethora of endogenous and synthetic compounds. Like in AHR, the pliable PAS-B cavity of MET adjusts to diverse ligands and binds them through similar mechanisms. Despite recent progress, we only begin to discern agonist-induced conformational shifts within the PAS-B domain, with the ultimate goal of understanding how these localized changes stimulate the assembly of the active JHR complex and, thus, fully grasp the mechanism of JHR signaling.

Faculty of Science University of South Bohemia Ceske Budejovice 37005 Czech Republic

Institute of Entomology Biology Center of the Czech Academy of Sciences Ceske Budejovice 37005 Czech Republic

Institute of Entomology Biology Center of the Czech Academy of Sciences Ceske Budejovice 37005 Czech Republic; Faculty of Science University of South Bohemia Ceske Budejovice 37005 Czech Republic

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MET goes to the membrane-A new place for the "old" juvenile hormone receptor