Trimetallic nanocomposites developed for efficient in vivo bimodal imaging via fluorescence and magnetic resonance
Language English Country Great Britain, England Media electronic
Document type Journal Article
PubMed
39072712
DOI
10.1039/d4tb00655k
Knihovny.cz E-resources
- MeSH
- Cell Line MeSH
- Contrast Media chemistry MeSH
- Humans MeSH
- Magnetic Resonance Imaging * methods MeSH
- Mice MeSH
- Nanocomposites * chemistry MeSH
- Optical Imaging MeSH
- Silver * chemistry MeSH
- Particle Size MeSH
- Cell Survival drug effects MeSH
- Gold * chemistry MeSH
- Animals MeSH
- Check Tag
- Humans MeSH
- Mice MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Contrast Media MeSH
- Silver * MeSH
- Gold * MeSH
Despite several attempts, in vivo bimodal imaging still represents a challenge. Generally, it is accepted that dual-modality in imaging can improve sensitivity and spatial resolution, namely, when exploiting fluorescence (FI) and magnetic resonance imaging (MRI), respectively. Here, a newly developed combination of (i) protein-protected luminescent Au-Ag nanoclusters (LGSN) manifesting themselves by fluorescent emission at 705 nm and (ii) superparamagnetic iron oxide nanoparticles (SPION) embedded within the same protein and creating contrast in MR images, has been investigated in phantoms and applied for in vivo bimodal imaging of a mouse as a proof of principle. Unique LGSN-SPION nanocomposites were synthesized in a specific sequential one-pot green preparation procedure and characterized thoroughly using many physicochemical experimental techniques. The influence of LGSN-SPION samples on the viability of healthy cells (RPE-1) was tested using a calcein assay. Despite the presence of Ag (0.12 mg mL-1), high content of Au (above 0.75 mg mL-1), and moderate concentrations of Fe (0.24 mg mL-1), LGSN-SPION samples (containing approx. 15 mg mL-1 of albumin) were revealed as biocompatible (cell viability above 80%). Simultaneously, these concentration values of all components in the LGSN-SPION nanocomposite were used for achieving both MRI and fluorescence signals in phantoms as well as in a living mouse with sufficiently high resolution. Thus, the LGSN-SPION samples can serve as new efficient bimodal FI and MRI probes for in vivo imaging.
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