Quantitative measurement of light intensity is a key step in ensuring the reliability and the reproducibility of scientific results in many fields of physics, biology, and chemistry. The protocols presented so far use various photoactive properties of manufactured materials. Here, leaves are introduced as an easily accessible green material to calibrate light intensity. The measurement protocol consists in monitoring the chlorophyll fluorescence of a leaf while it is exposed to a jump of constant light. The inverse of the characteristic time of the initial chlorophyll fluorescence rise is shown to be proportional to the light intensity received by the leaf over a wide range of wavelengths and intensities. Moreover, the proportionality factor is stable across a wide collection of plant species, which makes the measurement protocol accessible to users without prior calibration. This favorable feature is finally harnessed to calibrate a source of white light from exploiting simple leaves collected from a garden.

Department of Biophysics Faculty of Science Palacký University Olomouc 77900 Czech Republic

Institut de biologie de l'École normale supérieure École normale supérieure CNRS INSERM Université PSL Paris 75005 France

Jardin des Plantes de Paris Museum National d'Histoire Naturelle Paris 75005 France

PASTEUR Département de chimie École normale supérieure PSL University Sorbonne Université CNRS Paris 75005 France

Sony Computer Science Laboratories Paris 75005 France

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