Wildlife as Reservoirs of Encephalitozoon Cuniculi and Encephalitozoon Hellem and Molecular Genotyping of Encephalitozoon spp. in Small Mammals in the Czech Republic
Jazyk angličtina Země Švýcarsko Médium print-electronic
Typ dokumentu časopisecké články
PubMed
39356424
PubMed Central
PMC11649836
DOI
10.1007/s11686-024-00920-0
PII: 10.1007/s11686-024-00920-0
Knihovny.cz E-zdroje
- Klíčová slova
- Encephalitozoonosis, Genotyping, Urban area, Wildlife, Zoonosis,
- MeSH
- divoká zvířata * parazitologie MeSH
- Encephalitozoon cuniculi genetika izolace a purifikace klasifikace MeSH
- Encephalitozoon * genetika izolace a purifikace klasifikace MeSH
- encephalitozoonóza * veterinární epidemiologie parazitologie mikrobiologie MeSH
- genotyp * MeSH
- hlodavci * parazitologie MeSH
- hmyzožravci parazitologie MeSH
- játra parazitologie MeSH
- mozek parazitologie MeSH
- polymerázová řetězová reakce MeSH
- zdroje nemoci * parazitologie MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Geografické názvy
- Česká republika MeSH
PURPOSE: Parasites of genus Encephalitozoon are well known pathogens of domestic animals however less attention was paid to its spread among wildlife that can play an important role of reservoir of infection. The aim of the study was to conduct molecular detection and genotype characterization of Encephalitozoon spp. in wild small mammals trapped in localities both near to and at a large distance from residential areas. METHODS: In total, 300 wild small mammals (274 Rodentia and 26 Eulipotyphla) were trapped in 41 localities of the Czech Republic and tested by nested PCR for Encephalitozoon spp. RESULTS: The DNA of Encephalitozoon spp. was proved in tissues (brain or liver) of 11% (32/300) of animals. There was a statistically significant difference (p < 0.001) in positivity among animal species with the most infected species Micromys minutus (50%, 4/8) and Myodes glareolus (17%, 9/53). There was also statistically significant difference (p < 0.001) between localities with the higher positivity (29%, 12/42) in localities near to residential areas, compared to localities with a large distance from residential areas (8%, 20/258). Sex and age of wild small mammals did not have effect on their positivity. Genotyping analysis revealed E. cuniculi genotype II in 22 samples and E. hellem genotype 1 A in one sample. CONCLUSION: This study brings new information on the molecular characterization of Encephalitozoon spp. isolated from wild small mammals trapped in two different areas (localities in near to residential areas and localities with a large distance from residential areas).
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