Spirochetal bacteria isolated from arthropods of the genera Culex and Aedes are termed BR149, BR151 (Entomospira culicis), BR193 (Entomospira entomophila), and BR208 (Entomospira nematocerorum). Genome sizes assembled from Illumina MiSeq and Oxford Nanopore reads varied between 1.67 and 1.78 Mb containing three to six plasmids. GC content ranged from 38.5% to 45.76%.

Biology Centre Czech Academy of Sciences Institute of Parasitology České Budějovice Czech Republic

Cell and Systems Biology Department University of Toronto Toronto Canada

Faculty of Science University of South Bohemia in Česke Budějovice České Budějovice Czech Republic

Institute of Vertebrate Biology v v i Czech Academy of Sciences Brno Czech Republic

National Reference Center for Borreliosis Bavarian Health and Food Safety Authority Oberschleissheim Germany

Programa de Genómica Evolutiva Centro de Ciencias Genómicas Universidad Nacional Autónoma de México Cuernavaca Morelos Mexico

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Sikutová S, Halouzka J, Mendel J, Knoz J, Rudolf I. 2010. Novel spirochetes isolated from mosquitoes and black flies in the Czech Republic. J Vector Ecol 35:50–55. doi:10.1111/j.1948-7134.2010.00057.x PubMed DOI

Graña-Miraglia L, Sikutova S, Vancová M, Bílý T, Fingerle V, Sing A, Castillo-Ramírez S, Margos G, Rudolf I. 2020. Spirochetes isolated from arthropods constitute a novel genus Entomospira genus novum within the order Spirochaetales. Sci Rep 10:17053. doi:10.1038/s41598-020-74033-9 PubMed DOI PMC

Hepner S, Kuleshov K, Tooming-Kunderud A, Alig N, Gofton A, Casjens S, Rollins RE, Dangel A, Mourkas E, Sheppard SK, Wieser A, Hübner J, Sing A, Fingerle V, Margos G. 2023. A high fidelity approach to assembling the complex Borrelia genome. BMC Genomics 24:401. doi:10.1186/s12864-023-09500-4 PubMed DOI PMC

Margos G, Hepner S, Mang C, Marosevic D, Reynolds SE, Krebs S, Sing A, Derdakova M, Reiter MA, Fingerle V. 2017. Lost in plasmids: next generation sequencing and the complex genome of the tick-borne pathogen Borrelia burgdorferi. BMC Genomics 18:422. doi:10.1186/s12864-017-3804-5 PubMed DOI PMC

Bankevich A, Nurk S, Antipov D, Gurevich AA, Dvorkin M, Kulikov AS, Lesin VM, Nikolenko SI, Pham S, Prjibelski AD, Pyshkin AV, Sirotkin AV, Vyahhi N, Tesler G, Alekseyev MA, Pevzner PA. 2012. SPAdes: a new genome assembly algorithm and its applications to single-cell sequencing. J Comput Biol 19:455–477. doi:10.1089/cmb.2012.0021 PubMed DOI PMC

Parks DH, Imelfort M, Skennerton CT, Hugenholtz P, Tyson GW. 2015. CheckM: assessing the quality of microbial genomes recovered from isolates, single cells, and metagenomes. Genome Res 25:1043–1055. doi:10.1101/gr.186072.114 PubMed DOI PMC

Schwengers O, Barth P, Falgenhauer L, Hain T, Chakraborty T, Goesmann A. 2020. Platon: identification and characterization of bacterial plasmid contigs in short-read draft assemblies exploiting protein sequence-based replicon distribution scores. Microb Genom 6:mgen000398. doi:10.1099/mgen.0.000398 PubMed DOI PMC

Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ. 1990. Basic local alignment search tool. J Mol Biol 215:403–410. doi:10.1016/S0022-2836(05)80360-2 PubMed DOI

Wick RR, Schultz MB, Zobel J, Holt KE. 2015. Bandage: interactive visualization of de novo genome assemblies. Bioinformatics 31:3350–3352. doi:10.1093/bioinformatics/btv383 PubMed DOI PMC

Antipov D, Hartwick N, Shen M, Raiko M, Lapidus A, Pevzner PA. 2016. plasmidSPAdes: assembling plasmids from whole genome sequencing data. Bioinformatics 32:3380–3387. doi:10.1093/bioinformatics/btw493 PubMed DOI