Equine neosporosis is an intracellular protozoan disease with a global distribution, affecting a diverse range of warm-blooded animals. Neospora caninum Dubey, Carpenter, Speer, Topper et Uggla, 1988 is associated with foetal loss, neurological disease and abortion in equids. No information was available regarding equine N. caninum infection among equids in Iraq. Thus, the aim of this study was to determine the prevalence rate of N. caninum in equines by using a competitive enzyme-linked immunosorbent assay (c-ELISA). A total of 329 blood samples randomly selected from equines, comprising 268 horses and 61 donkeys were examined. The seroprevalence rate of N. caninum was determined as 46% (28/61) for donkeys and 24% (64/268) for horses. The prevalence of N. caninum indicated a significantly higher risk of infection in donkeys compared to horses (P < 0.001). However, the odds of N. caninum infection in draught equids were 8.2 times greater than other equids with a significant difference (P < 0.001). The current study revealed no significant differences in the prevalence of N. caninum across various genders, breeds, clinical statuses, disease histories and among equids that had contact with dogs. While outdoor feeding and mixed (grazing), showed a significant difference (P = 0.003) and (P = 0.75), respectively, in the presence of antibodies against N. caninum compared to indoor feeding (stable). Moreover, the odds of infection in equids with a history of late abortion were 4.8 times higher than those without such a history of abortion (2.20-10.56) with statistical significance (P < 0.001).

College of Agricultural Engineering Sciences Department of Fish Resources and Aquatic Animals Salahaddin University Erbil Erbil Iraq *Address for correspondence Khalid Jabar Aziz College of Veterinary Medicine Salahaddin University Erbil Erbil Iraq; Email ORCID 0000 0002 6662 3863

College of Education Biology Department Salahaddin University Erbil Erbil Iraq

College of Veterinary Medicine Salahaddin University Erbil Erbil Iraq

College of Veterinary Medicine University of Duhok Duhok Iraq

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Alshammari A., Gattan H.S., Marzok M., Selim A. 2023: Seroprevalence and risk factors for Neospora spp. infection in equine in Egypt. Sci. Rep. 13: 20242. PubMed DOI

Bártová E., Machačová T., Sedlák K., Budíková M., Mariani U., Veneziano V. 2015: Seroprevalence of antibodies of Neospora spp. and Toxoplasma gondii in horses from southern Italy. Folia Parasitol. 62: 043. PubMed DOI

Bártová E., Sedlák K., Syrová M., Literák I. 2010: Neospora spp. and Toxoplasma gondii antibodies in horses in the Czech Republic. Parasitol. Res. 107: 783-785. PubMed DOI

Ciaramella P., Corona M., Cortese L., Piantedosi D., Santoro D., Di Loria A., Rigato R. 2004: Seroprevalence of Neospora spp. in asymptomatic horses in Italy. Vet. Parasitol. 123: 11-15. PubMed DOI

Cruz I., Vinhas A.R., Dubey J.P., Cardoso L., Cotovio M., Lopes A.P. 2019: First report of antibodies to Neospora spp. in horses from Portugal. Rev. Bras. Parasitol. Vet. 28: 161-163. PubMed DOI

Dangoudoubiyam S., Oliveira J.B., Víquez C., Gómez-García A., González O., Romero J.J., Kwok O.C., Dubey J.P., Howe D.K. 2011: Detection of antibodies against Sarcocystis neurona, Neospora spp., and Toxoplasma gondii in horses from Costa Rica. J. Parasitol. 97: 522-524. PubMed DOI

Dubey J.P. 2003: Review of Neospora caninum and neosporosis in animals. Kor. J. Parasitol. 41: 1. PubMed DOI

Dubey J.P., Hemphill A., Calero-Bernal R., Schares G. 2017: Neosporosis in Animals. CRC Press, Boca Raton, 548 pp. DOI

Dubey J.P., Lindsay D.S. 1996: A review of Neospora caninum and neosporosis. Vet. Parasitol. 67: 1-59. PubMed DOI

Dubey J.P., Mitchell S.M., Morrow J.K., Rhyan J.C., Stewart L.M., Granstrom D.E., Romand S., Thulliez P., Saville W.J., Lindsay D.S. 2003: Prevalence of antibodies to Neospora caninum, Sarcocystis neurona, and Toxoplasma gondii in wild horses from central Wyoming. J. Parasitol. 89: 716-720. PubMed DOI

Dubey J.P., Schares G. 2011: Neosporosis in animals-the last five years. Vet. Parasitol. 180: 90-108. PubMed DOI

Dubey J.P., Schares G., Ortega-Mora L. 2007: Epidemiology and control of neosporosis and Neospora caninum. Clin. Microbiol. Rev. 20: 323-67. PubMed DOI

Gennari S.M., Pena H.F.D.J., Lindsay D.S., Lopes M.G., Soares H.S., Cabral A.D., Vitaliano S.N., Amaku M. 2016: Prevalence of antibodies against Neospora spp. and Sarcocystis neurona in donkeys from northeastern Brazil. Rev. Bras. Parasitol. Vet. 25: 109-111. PubMed DOI

Gharekhani J., Heidari H. 2014: Serology based comprehensive study of Neospora infection in domestic animals in Hamedan province, Iran. J. Adv. Vet. Anim. Res. 1: 119-124. DOI

Gharekhani J., Tavoosidana G.R., Naderisefat G.R. 2013: Seroprevalence of Neospora infection in horses and donkeys in Hamedan province, Western Iran. Vet. World 6: 620. DOI

Hiasa J., Kohara J., Nishimura M., Xuan X., Tokimitsu H., Nishikawa Y. 2012: ELISAs based on rNcGRA7 and rNcSAG1 antigens as an indicator of Neospora caninum activation. Vet. Parasitol. 187: 379-385. PubMed DOI

Hoane J.S., Gennari S.M., Dubey J.P., Ribeiro M.G., Borges A.S., Yai L.E., Aguiar D.M., Cavalcante G.T., Bonesi G.L., Howe D.K. 2006: Prevalence of Sarcocystis neurona and Neospora spp. infection in horses from Brazil based on presence of serum antibodies to parasite surface antigen. Vet. Parasitol. 136: 155-159. PubMed DOI

Jakubek E.B., Lundén A., Uggla A. 2006: Seroprevalences of Toxoplasma gondii and Neospora sp. infections in Swedish horses. Vet. Parasitol. 138: 194-199. PubMed DOI

Javanmardi E., Majidiani H., Shariatzadeh S.A., Anvari D., Shamsinia S., Ghasemi E., Kordi B., Shams M. 2020: Global seroprevalence of Neospora spp. in horses and donkeys: a systematic review and meta-analysis. Vet. Parasitol. 288: 109299. PubMed DOI

Karatepe M., Karatepe B. 2012: Investigation of seroprevalence of Neospora spp. in horses in Niğde province (Turkey). Kafkas Univ. Vet. Fak. Derg. A39-A42.

Kligler E.B., Shkap V., Baneth G., Mildenberg Z., Steinman A. 2007: Seroprevalence of Neospora spp. among asymptomatic horses, aborted mares and horses demonstrating neurological signs in Israel. Vet. Parasitol. 148: 109-113. PubMed DOI

Leszkowicz Mazuz M., Mimoun L., Schvartz G. Tirosh-Levy S., Savitzki I., Edery N., Blum S.E., Baneth G., Pusterla N., Steinman A. 2020: Detection of Neospora caninum infection in aborted equine fetuses in Israel. Pathogens 9: 962. PubMed DOI

Marsh A.E., Howe D.K., Wang G., Barr B.C., Cannon N., Conrad P.A. 1999: Differentiation of Neospora hughesi from Neospora caninum based on their immunodominant surface antigen, SAG1 and SRS2. Int. J. Parasitol. 29: 1575-1582. PubMed DOI

Pagmadulam B., Myagmarsuren P., Fereig R.M., Igarashi M., Yokoyama N., Battsetseg B., Nishikawa Y. 2018: Seroprevalence of Toxoplasma gondii and Neospora caninum infections in cattle in Mongolia. Vet. Parasitol. Reg. Stud. Rep. 14: 11-17. PubMed DOI

Pitel P.H., Pronost S., Chatagnon G., Tainturier D., Fortier G., Ballet J.J. 2001: Neosporosis in bovine dairy herds from the west of France: detection of Neospora caninum DNA in aborted fetuses, seroepidemiology of N. caninum in cattle and dogs. Vet. Parasitol. 102: 269-277. PubMed DOI

Santolaria Blasco P., Almería S., Martínez Bello D., Nogareda C., Mezo M., Gonzalez Warleta M., Pabón M., López Gatius F., Yániz J.L., Castro Hermida J.A. 2011: Different humoral mechanisms against Neospora caninum infection in purebreed and crossbreed beef/dairy cattle pregnancies. Vet. Parasitol. 178: 70-76. PubMed DOI

Saqib M., Hussain M.H., Sajid M.S., Mansoor M.K., Asi M.N., Fadya A.A., Zohaib A., Sial A.U.R., Muhammad G., Ullah I. 2015: Sero-epidemiology of equine toxoplasmosis using a latex agglutination test in the three metropolises of Punjab, Pakistan. Trop. Biomed. 32: 276-285.

Selim A., Marawan M. A., Ali A.-F., Manaa E., AbouelGhaut H. A. 2020a: Seroprevalence of bovine leukemia virusin cattle, buffalo, and camel in Egypt. Trop. Anim. Health Prod. 52: 1207-1210. PubMed DOI

Selim A., Radwan A., Arnaout F., Khater H. 2020b: The recent update of the situation of West Nile fever among equids in Egypt after three decades of missing information. Pak. Vet. J. 40: 100. DOI

Talafha A.Q., Abutarbush S.M., Rutley D.L. 2015: Seroprevalence and potential risk factors associated with Neospora spp. infection among asymptomatic horses in Jordan. Kor. J. Parasitol. 53: 163. PubMed DOI

Tirosh-Levy S., Steinman A., Minderigiu A., Arieli O., Savitski I., Fleiderovitz L., Edery N., Schvartz G., Leszkowicz Mazuz M. 2020: High exposure to Toxoplasma gondii and Neospora spp. in donkeys in Israel: serological survey and case reports. Animals 10: 1921. PubMed DOI

Vanleeuwen J.A., Haddad J.P., Dohoo I.R., Keefe G.P., Tiwari A, Scott HM. 2010: Risk factors associated with Neospora caninum seropositivity in randomly sampled Canadian dairy cows and herds. Prev. Vet. Med. 93: 129-138. PubMed DOI

Vardeleon D., Marsh A.E., Thorne J.G., Loch W., Young R., Johnson P.J. 2001: Prevalence of Neospora hughesi and Sarcocystis neurona antibodies in horses from various geographical locations. Vet. Parasitol. 95: 273-282. PubMed DOI

Veronesi F., Diaferia M., Mandara M.T., Marenzoni M.L., Cittadini F., Piergili Fioretti D. 2008: Neospora spp. infection associated with equine abortion and/or stillbirth rate. Vet. Res. Commun. 32: 223-226. PubMed DOI

de Waal T. 2012: Advances in diagnosis of protozoan diseases. Vet. Parasitol. 189: 65-74. PubMed DOI

Zhou M., Cao S., Sevinc F., Sevinc M., Ceylan O., Liu M., et al. 2017: Enzyme-linked immunosorbent assays using recombinant TgSAG2 and NcSAG1 to detect Toxoplasma gondii and Neospora caninum-specific antibodies in domestic animals in Turkey. J. Vet. Med. Sci. 78: 1877-1881. PubMed DOI