Neospora spp. and Toxoplasma gondii antibodies in horses in the Czech Republic
Language English Country Germany Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Enzyme-Linked Immunosorbent Assay MeSH
- Fluorescent Antibody Technique, Indirect MeSH
- Coccidiosis epidemiology immunology veterinary MeSH
- Horses MeSH
- Latex Fixation Tests MeSH
- Horse Diseases epidemiology immunology parasitology MeSH
- Neospora immunology MeSH
- Antibodies, Protozoan blood MeSH
- Seroepidemiologic Studies MeSH
- Serum immunology MeSH
- Toxoplasma immunology MeSH
- Toxoplasmosis, Animal epidemiology immunology MeSH
- Animals MeSH
- Check Tag
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Geographicals
- Czech Republic epidemiology MeSH
- Names of Substances
- Antibodies, Protozoan MeSH
During January 2007, blood samples were collected from 552 healthy horses from nine different regions of the Czech Republic. Sera were tested for serum antibodies to Neospora caninum by a competitive-inhibition enzyme-linked immunosorbent assay and confirmed by an indirect fluorescent antibody test. The same samples were tested for serum antibodies against Toxoplasma gondii by a latex agglutination test. In total, 131 of 552 (24%) horses reacted positively for Neospora antibodies in competitive-inhibition enzyme-linked immunosorbent assay; seven of them had > or =50% of inhibition. Samples were confirmed in indirect fluorescence test, and only two samples were positive with final titres 50 and 100, while others were negative. Antibodies against T. gondii were found in 125 (23%) horses. This is the first serologic survey for Neospora spp. antibodies performed on horses in the Czech Republic.
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