Specialized or secondary metabolites are small molecules of biological origin, often showing potent biological activities with applications in agriculture, engineering and medicine. Usually, the biosynthesis of these natural products is governed by sets of co-regulated and physically clustered genes known as biosynthetic gene clusters (BGCs). To share information about BGCs in a standardized and machine-readable way, the Minimum Information about a Biosynthetic Gene cluster (MIBiG) data standard and repository was initiated in 2015. Since its conception, MIBiG has been regularly updated to expand data coverage and remain up to date with innovations in natural product research. Here, we describe MIBiG version 4.0, an extensive update to the data repository and the underlying data standard. In a massive community annotation effort, 267 contributors performed 8304 edits, creating 557 new entries and modifying 590 existing entries, resulting in a new total of 3059 curated entries in MIBiG. Particular attention was paid to ensuring high data quality, with automated data validation using a newly developed custom submission portal prototype, paired with a novel peer-reviewing model. MIBiG 4.0 also takes steps towards a rolling release model and a broader involvement of the scientific community. MIBiG 4.0 is accessible online at https://mibig.secondarymetabolites.org/.

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Zobrazit více v PubMed

Dewick  P.M.  Medicinal Natural Products: A Biosynthetic Approach. 2009; 3rd ednHoboken, NJ: Wiley-Blackwell.

Newman  D.J., Cragg  G.M.  Natural products as sources of new drugs over the nearly four decades from 01/1981 to 09/2019. J. Nat. Prod.  2020; 83:770–803. PubMed

Fischbach  M.A., Walsh  C.T., Clardy  J.  The evolution of gene collectives: how natural selection drives chemical innovation. Proc. Natl Acad. Sci. U.S.A.  2008; 105:4601–4608. PubMed PMC

Medema  M.H., de Rond  T., Moore  B.S.  Mining genomes to illuminate the specialized chemistry of life. Nat. Rev. Genet.  2021; 22:553–571. PubMed PMC

Blin  K., Shaw  S., Augustijn  H.E., Reitz  Z.L., Biermann  F., Alanjary  M., Fetter  A., Terlouw  B.R., Metcalf  W.W., Helfrich  E.J.N.  et al. .  antiSMASH 7.0: new and improved predictions for detection, regulation, chemical structures and visualisation. Nucleic Acids Res.  2023; 51:W46–W50. PubMed PMC

van Heel  A.J., de Jong  A., Song  C., Viel  J.H., Kok  J., Kuipers  O.P.  BAGEL4: a user-friendly web server to thoroughly mine RiPPs and bacteriocins. Nucleic Acids Res.  2018; 46:W278–W281. PubMed PMC

Skinnider  M.A., Johnston  C.W., Gunabalasingam  M., Merwin  N.J., Kieliszek  A.M., MacLellan  R.J., Li  H., Ranieri  M.R.M., Webster  A.L.H., Cao  M.P.T.  et al. .  Comprehensive prediction of secondary metabolite structure and biological activity from microbial genome sequences. Nat. Commun.  2020; 11:6058. PubMed PMC

Merwin  N.J., Mousa  W.K., Dejong  C.A., Skinnider  M.A., Cannon  M.J., Li  H., Dial  K., Gunabalasingam  M., Johnston  C., Magarvey  N.A.  DeepRiPP integrates multiomics data to automate discovery of novel ribosomally synthesized natural products. Proc. Natl Acad. Sci. U.S.A.  2020; 117:371–380. PubMed PMC

Liu  M., Li  Y., Li  H.  Deep learning to predict the biosynthetic gene clusters in bacterial genomes. J. Mol. Biol.  2022; 434:167597. PubMed

Hannigan  G.D., Prihoda  D., Palicka  A., Soukup  J., Klempir  O., Rampula  L., Durcak  J., Wurst  M., Kotowski  J., Chang  D.  et al. .  A deep learning genome-mining strategy for biosynthetic gene cluster prediction. Nucleic Acids Res.  2019; 47:e110. PubMed PMC

Sanchez  S., Rogers  J.D., Rogers  A.B., Nassar  M., McEntyre  J., Welch  M., Hollfelder  F., Finn  R.D.  Expansion of novel biosynthetic gene clusters from diverse environments using SanntiS. 2023; bioRxiv doi:02 June 2023, preprint: not peer reviewed10.1101/2023.05.23.540769. DOI

Carroll  L.M., Larralde  M., Fleck  J.S., Ponnudurai  R., Milanese  A., Cappio  E., Zeller  G.  Accurate de novo identification of biosynthetic gene clusters with GECCO. 2021; bioRxiv doi:04 May 2021, preprint: not peer reviewed10.1101/2021.05.03.442509. DOI

Kalmer  T.L., Ancajas  C.M.F., Cheng  Z., Oyedele  A.S., Davis  H.L., Walker  A.S.  Assessing the ability of ChatGPT to extract natural product bioactivity and biosynthesis data from publications. 2024; bioRxiv doi:02 August 2024, preprint: not peer reviewed10.1101/2024.08.01.606186. DOI

Rajan  K., Zielesny  A., Steinbeck  C.  DECIMER: towards deep learning for chemical image recognition. J. Cheminform.  2020; 12:65. PubMed PMC

Medema  M.H., Kottmann  R., Yilmaz  P., Cummings  M., Biggins  J.B., Blin  K., de Bruijn  I., Chooi  Y.H., Claesen  J., Coates  R.C.  et al. .  Minimum information about a biosynthetic gene cluster. Nat. Chem. Biol.  2015; 11:625–631. PubMed PMC

Kautsar  S.A., Blin  K., Shaw  S., Navarro-Muñoz  J.C., Terlouw  B.R., van der Hooft  J.J.J., van Santen  J.A., Tracanna  V., Suarez Duran  H.G., Pascal Andreu  V.  et al. .  MIBiG 2.0: a repository for biosynthetic gene clusters of known function. Nucleic Acids Res.  2020; 48:D454–D458. PubMed PMC

Terlouw  B.R., Blin  K., Navarro-Muñoz  J.C., Avalon  N.E., Chevrette  M.G., Egbert  S., Lee  S., Meijer  D., Recchia  M.J.J., Reitz  Z.L.  et al. .  MIBiG 3.0: a community-driven effort to annotate experimentally validated biosynthetic gene clusters. Nucleic Acids Res.  2023; 51:D603–D610. PubMed PMC

Dell  M., Dunbar  K.L., Hertweck  C.  Ribosome-independent peptide biosynthesis: the challenge of a unifying nomenclature. Nat. Prod. Rep.  2022; 39:453–459. PubMed

Zdouc  M.M., Meijer  D., Biermann  F., Holme  J., Korenskaia  A., Lien  A., Louwen  N.L.L., Navarro-Muñoz  J.C., Nguyen  G.-S., Rutz  A.  et al. .  The Minimum Information about a Tailoring Enzyme/Maturase data standard for capturing natural product biosynthesis. 2024; ChemRxiv doi:09 April 2024, preprint: not peer reviewed10.26434/chemrxiv-2024-78mtl. DOI

Jones  M.R., Pinto  E., Torres  M.A., Dörr  F., Mazur-Marzec  H., Szubert  K., Tartaglione  L., Dell’Aversano  C., Miles  C.O., Beach  D.G.  et al. .  CyanoMetDB, a comprehensive public database of secondary metabolites from cyanobacteria. Water Res.  2021; 196:117017. PubMed