Reduced lipid and glucose oxidation and reduced lipid synthesis in AMPKα2-/- myotubes
Jazyk angličtina Země Anglie, Velká Británie Médium print-electronic
Typ dokumentu časopisecké články
- Klíčová slova
- AMPK, energy metabolism, oxidative phosphorylation, skeletal muscle,
- MeSH
- energetický metabolismus MeSH
- glukosa * metabolismus MeSH
- kosterní svalová vlákna * metabolismus cytologie MeSH
- kosterní svaly metabolismus MeSH
- kultivované buňky MeSH
- mastné kyseliny metabolismus MeSH
- metabolismus lipidů * MeSH
- mitochondrie metabolismus MeSH
- myši knockoutované MeSH
- myši MeSH
- oxidace-redukce MeSH
- proteinkinasy aktivované AMP * genetika metabolismus nedostatek MeSH
- zvířata MeSH
- Check Tag
- myši MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- glukosa * MeSH
- mastné kyseliny MeSH
- Prkaa2 protein, mouse MeSH Prohlížeč
- proteinkinasy aktivované AMP * MeSH
Adenosine 5'-monophosphate (AMP)-activated protein kinase (AMPK) plays a crucial role in regulation of metabolic homeostasis. To understand the role of the catalytic α2 subunit of AMPK in skeletal muscle energy metabolism, myotube cultures were established from AMPKα2+/+ and AMPKα2-/- mice. Myotubes from AMPKα2-/- mice had lower basal oleic acid and glucose oxidation compared to myotubes from AMPKα2+/+ mice. However, the relative response to mitochondrial uncoupling was increased for oleic acid oxidation. Incorporation of acetate into lipids was also lower in myotubes from AMPKα2-/- mice. Proteomics analysis revealed that AMPKα2-/- myotubes had upregulated pathways related to mitochondrial function and fatty acid oxidation, and decreased pathways related to fatty acid biosynthesis. In conclusion, ablation of AMPKα2 catalytic subunit in skeletal muscle cells resulted in reduced basal oxidation of glucose and fatty acids, however upregulated pathways related to mitochondrial function and fatty acid oxidation and reduced lipid formation.
Citace poskytuje Crossref.org
