Mycoplasma hyopneumoniae and Actinobacillus pleuropneumoniae are causative agents of the porcine respiratory disease complex. However, information on the prevalence of these pathogens in wild boars is scarce. This study aimed to investigate the presence of antibodies to Mycoplasma hyopneumoniae and Actinobacillus pleuropneumoniae in wild boars in Serbia. In this study 253 serum samples from wild boars were tested for antibodies to Mycoplasma hyopneumoniae and Actinobacillus pleuropneumonia using the ELISA assay. The overall seroprevalence rates of Mycoplasma hyopneumoniae and Actinobacillus pleuropneumoniae were 4.2% and 56.9%, respectively. Antibodies to both pathogens were detected in 20 sera samples (7.9%). The prevalence of wild boars that were seropositive for Mycoplasma hyopneumoniae differed with age and ranged from 10.7% to 33.3%, and for Actinobacillus pleuropneumoniae, it ranged from 51.8% to 83.3%. Wild boars are hard to control and are considered a high-risk infection source for outdoor and backyard pigs and eventually for commercial indoor farms as well. Thus, the result of this first serosurvey in Serbia should raise awareness of the importance of wild boars as potential reservoirs of bacterial pathogens such as Mycoplasma hyopneumoniae and Actinobacillus pleuropneumoniae. Our data revealed the circulation of both pathogens in wild boars in Serbia, drawing attention to the potential health risk they present for domestic swine health.

Scientific Institute of Veterinary Medicine of Serbia Belgrade Serbia

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Angjelovski B, Janevski AL, Atanaskova-Petrov E, Marin Orenga C, Bojkovski J. Serological profile and pleurisy lesions associated with Actinobacillus pleuropneumoniae in pig farms in North Macedonia. Vet Gl. 2023 Feb;77(2): 137-48.

Batista Linhares M, Belloy L, Origgi FC, Lechner I, Segner H, Ryser-Degiorgis MP. Investigating the role of free-ranging wild boar (Sus scrofa) in the re-emergence of enzootic pneumonia in domestic pig herds: A pathological, prevalence and risk-factor study. PLoS One. 2015 Mar;10(3). PubMed PMC

Bojkovski J, Stankovic B, Prodanov-Radulovic J, Maletic M, Vakanjac S, Zdravkovic N. Procena biosigurnosnih mera na komercijalnim farmama svinja [Assessment of iosecurity measures on commercial pig farms]. Vet J Republ Srpska. 2018 Dec;18(2):463-86. Serbian.

de Souza TCGD, Silva VS, Mores MAZ, Kramer B, Leme RA, da Silva Porto G, Alfieri AA. Mycoplasma hyopneumoniae in free-living wild boars in Parana, Brazil. Braz J Microbiol. 2021 Sep;52(3):1581-7. PubMed PMC

Deffner P, Maurer R, Cvjetkovic V, Sipos W, Krejci R, Ritzmann M, Eddicks M. Cross-sectional study on the in-herd prevalence of Mycoplasma hyopneumoniae at different stages of pig production. Vet Rec. 2022 Jul;191(7). PubMed

Elbers AR, Dekkers LJ, Van der Giessen JW. Sero-surveillance of wild boar in the Netherlands, 1996–1999. Rev Sci Tech Off Int Epizooty. 2000 Sep;19(3):848-54. PubMed

Halli O, Ala-Kurikka E, Wallgren P, Heinonen M. Actinobacillus pleuropneumoniae seroprevalence in farmed wild boars in Finland. J Zoo Wildl Med. 2014 Dec;45(4):813-8. PubMed

Leal Zimmer FM, Paes JA, Zaha A, Ferreira HB. Pathogenicity and virulence of Mycoplasma hyopneumoniae. Virulence. 2020 Nov;11:1600-22. PubMed PMC

Liang QL, Zou Y, Gao YH, Nie LB, Zhang XX, Hu GX, Riu D, Zhu XQ. First report of Mycoplasma hyopneumoniae seroprevalence in farmed wild boars in China. Acta Trop. 2018 Oct;182:212-4. PubMed

Maes D, Segales J, Meyns T, Sibila M, Pieters M, Haesebrouck F. Control of Mycoplasma hyopneumoniae infections in pigs. Vet Microbiol. 2008 Nov;126(4):297-309. PubMed PMC

Makovska I, Dhaka P, Chantziaras I, Pessoa J, Dewulf J. The role of wildlife and pests in the transmission of pathogenic agents to domestic pigs: A systematic review. Animals. 2023 May;13(11):1830. PubMed PMC

Marinou KA, Papatsiros VG, Gkotsopoulos EK, Odatzoglou PK, Athanasiou LV. Exposure of extensively farmed wild boars (Sus scrofa scrofa) to selected pig pathogens in Greece. Vet Q. 2015 Jun;35(2):97-101. PubMed

Ministry of Agriculture, Forestry and Water Management. Law on Game and Hunting. Official Gazette of the Republic of Serbia. 2010;18/10.

Ministry of Agriculture, Forestry and Water Economy. Plan and method of monitoring of classical swine fever and African swine fever in wild boar in 2022 [Internet]. 2023. [cited 2024 Mar 9]. Available from: https://www.vet.minpolj.gov.rs/storage/2023/06/Plan-i-nacin-vrsenja-monitoringa-na-KKS-i-AKS-kod-divljih-svinja-u-2022.-i-2023.godinu.pdf.

Neskovic M, Ristic B, Dosenovic R, Grubac S, Petrovic T, Prodanov-Radulovic J, Polacek V. African swine fever outbreak investigation on large commercial pig farm in Serbia. Acta Vet Belgrade. 2021 May;71(2):219-29.

Pepovich R, Hristov K, Nikolov B, Genova K, Ivanova E. Seroprevalence of Actinobacillus pleuropneumoniae infection in pigs from Bulgaria. Bulg J Vet Med. 2022 Aug; 25(2):333-8.

Prodanov-Radulovic J, Dosen R, Pusic I, Petrovic T, Apic J, Stojanov I, Polacek V. Emergence of pseudorabies virus (morbus aujeszky) infection at large swine farms in AP Vojvodina (Serbia). Contemp Agric. 2015 Apr;64(1-2):105-11.

Prodanov-Radulovic J, Mirceta J, Djurdjevic B, Lazic S, Aleksic-Kovacevic S, Petrovic J, Polacek V. African swine fever outbreak in an enclosed wild boar hunting ground in Serbia. Pathogens. 2023 Jan;12(5):691. PubMed PMC

Prodanov-Radulovic J, Vucicevic I, Polacek V, Aleksic-Kovacevic S. Current swine respiratory diseases morphology in intensive swine production in Serbia. Acta Vet Belgrade. 2020 Feb;70(1):1-36.

Reiner G, Fresen C, Bronnert S, Haack I, Willems H. Prevalence of Actinobacillus pleuropneumoniae infection in hunted wild boars (Sus scrofa) in Germany. J Wildl Dis. 2010 Jun;46(2):551-5. PubMed

Risco D, Serrano E, Fernandez-Llario P, Cuesta JM, Goncalves P, Garcia-Jimenez WL, Martinez R, Cerrato R, Velarde R, et al.. Severity of bovine tuberculosis is associated with co-infection with common pathogens in wild boar. PLoS One. 2014 Oct;9(10). PubMed PMC

Rycroft AN, Garside LH. Actinobacillus species and their role in animal disease. Vet J. 2000;159(1):18-36. PubMed

Sassu EL, Bosse JT, Tobias TJ, Gottschalk M, Langford PR, Hennig-Pauka I. Update on Actinobacillus pleuropneumoniae: Knowledge, gaps and challenges. Transbound Emerg Dis. 2017;65:72-90. PubMed

Savic B, Radanovic O, Jovicic D, Nesic K, Ivanovic S, Stevancevic O, Cvetocevic D, Kasagic D. Survey of infectious agents associated with porcine respiratory disease complex (PRDC) in Serbian swine herds using polymerase chain reaction (PCR) detection. Acta Vet Belgrade. 2015;65(1):79-88.

Sibila M, Mentaberre G, Boadella M, Huerta E, Casas-Diaz E, Vicente J, Segales J. Serological, pathological and polymerase chain reaction studies on Mycoplasma hyopneumoniae infection in the wild boar. Vet Microbiol. 2010;144(1-2):214-8. PubMed

Statistical Office of the Republic of Serbia. Numbers of wild boars [Internet]. 2021. [cited 2023 Nov 5]. Available from: https://data.stat.gov.rs/Home/Result/13040601?languageCode=sr-Latn.

Stringer OW, Bosse JT, Lacouture S, Gottschalk M, Fodor L, Angen O, Velazquez E, Penny P, Lei L, Langford PR, Li Y. Proposal of Actinobacillus pleuropneumoniae serovar 19, and reformulation of previous multiplex PCRs for capsule-specific typing of all known serovars. Vet Microbiol. 2021;255:109021. PubMed

Stukelj M, Toplak I, Vengust G. Prevalence of antibodies against selected pathogens in wild boars (Sus scrofa) in Slovenia. Slov Vet Res. 2014;51(1):21-7.

Tarvydas A, Belova O. Effect of wild boar (Sus scrofa L.) on forests, agricultural lands and population management in Lithuania. Diversity. 2022;14:801.

Torremorell M, Pijoan C, Janni K, Walker R, Joo HS. Airborne transmission of Actinobacillus pleuropneumoniae and porcine reproductive and respiratory syndrome virus in nursery pigs. Am J Vet Res. 1997;58(8):828-32. PubMed

Touloudi A, Valiakos G, Athanasiou LV, Birtsas P, Giannakopoulos A, Papaspyropoulos K, Kalaitzis C, Sokos C, Tsokana CN, Spyrou V, Petrovska L, Bilinis C. A serosurvey for selected pathogens in Greek European wild boar. Vet Rec Open. 2015;2(2):e000077. PubMed PMC

Vengust G, Valencak Z, Bidovec A. A serological survey of selected pathogens in wild boar in Slovenia. J Vet Med B. 2006;53(1):24-7. PubMed PMC

Zdravkovic N, Radanovic O, Savic B, Ninkovic M, Pavlovic I, Bojkovski J, Jezdimirovic N. Bakterijske infekcije u kompleksu respiratornih bolesti svinja [Bacterial infections in the complex of swine respiratory diseases]. In: Proceedings of the 28th Annual Meeting of Veterinary Doctors of the Republic of Srpska (Bosnia and Herzegovina); 2023 Jun 15-17; Trebinje, Bosnia and Herzegovina. Banja Luka (BA): Veterinary Chamber of the Republic of Srpska; 2023. p. 33.

Zutic J, Vojinovic D, Radosavljevic V. Seroprevalence of Actinobacillus pleuropneumoniae in swine originated from commercial farms in Serbia. Biotechn Anim Husb. 2014;30(2):295-302.