The rat lungworm Angiostrongylus cantonensis is a zoonotic metastrongyloid nematode, currently considered an emerging pathogen approaching Europe. In tropics and subtropics, it is an important food-borne neurotropic parasite of medical and veterinary importance. Sources of infection for mammals and birds include gastropod intermediate hosts and poikilothermic vertebrates (paratenic hosts). To evaluate the relevance of reptiles in the rat lungworm circulation, we performed an experimental series focused on long-term survival of third stage larvae (L3) of A. cantonensis in reptiles and potential of saurians to serve as a source of infection for further hosts. Twenty leopard geckos (Eublepharis macularius) were infected with varying doses of L3 (100, 1000, 10 000 larvae per animal). Live L3 were collected from all infected geckos (mostly in musculature and liver) euthanized 1-6 months after the infection and were proven to be infective for Wistar rats (definitive hosts). Three sacrificed geckos were subsequently fed to three corn snakes (Pantheropis guttatus) to test hypothesis of L3 infectivity for predators positioned higher in the food chain. Snakes were euthanized 1 month post-infection and live L3 were detected predominantly in the intestinal wall. The animals remained clinically healthy throughout the study. No reptiles showed significant changes in haematological and biochemical blood parameters, though elevated CK and GLDH were observed in most geckos in the group receiving higher infectious dose. This study highlights the significant potential of reptiles to play a crucial role in the circulation of metastrongyloid nematodes in food web and in their transmission to humans.

3rd Faculty of Medicine Charles University Prague Czech Republic

Clinical and Transplant Pathology Centre Institute for Clinical and Experimental Medicine Prague Czech Republic

Department of Botany and Zoology Masaryk University Brno Czech Republic

Department of Comparative Pathobiology College of Veterinary Medicine Purdue University Lafayette IN USA

Department of Pathology and Parasitology University of Veterinary Sciences Brno Brno Czech Republic

Department of Veterinary Sciences Czech University of Life Sciences Prague Czech Republic

Institute of Parasitology Biology Center of Czech Academy of Sciences Ceske Budejovice Czech Republic

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Anderson RC (2000). Nematode parasites of vertebrates: Their development and transmission (2nd). CABI Publishing.

Anettová L, Izquierdo-Rodriguez E, Foronda P, Baláž V, Novotný L and Modrý D (2022) Endemic lizard Gallotia galloti is a paratenic host of invasive Angiostrongylus cantonensis in Tenerife, Spain. Parasitology 149(7), 934–939. PubMed PMC

Ash LR (1968). The occurrence of Angiostrongylus cantonensis in frogs of New Caledonia with observations on paratenic hosts of metastrongyles. The Journal of Parasitology, 432–436. PubMed

Bels V, Paindavoine AS, Zghikh LN, Paulet E, Pallandre JP and Montuelle SJ (2019) Feeding in lizards: Form–function and complex multifunctional system. In Bels V and Whishaw IQ (eds.), Feeding Invertebrates: Evolution, Morphology, Behavior, Biomechanics. Cham: Springer International Publishing, 469–525.

Brockelman C, Chusatayanond W and Baidikul V (1976) Growth and localization of Angiostrongylus cantonensis in the molluscan host, Achatina fulica. The Southeast Asian Journal of Tropical Medicine and Public Health 1, 30–37. PubMed

Burns RE, Bicknese EJ, Qvarnstrom Y, DeLeon-Carnes M, Drew CP, Gardiner CH and Rideout BA (2014) Cerebral Angiostrongylus cantonensis infection in a captive African pygmy falcon (Polihierax semitorquatus) in southern California. Journal of Veterinary Diagnostic Investigation 26(5), 695–698. PubMed

Bush AO (2001). Parasitism: The diversity and ecology of animal parasites. Cambridge University Press.

Červená B, Modrý D, Fecková B, Hrazdilová K, Foronda P, Alonso AM, Lee R, Walker J, Niebuhr CN, Malik R, et al. (2019) Low diversity of Angiostrongylus cantonensis complete mitochondrial DNA sequences from Australia, Hawaii, French Polynesia and the Canary Islands revealed using whole genome next-generation sequencing. Parasites and Vectors 12(1), 1–13. PubMed PMC

Colella V, Giannelli A, Brianti E, Ramos RAN, Cantacessi C, Dantas-Torres F and Otranto D (2015) Feline lungworms unlock a novel mode of parasite transmission. Scientific Reports 5(1), 13105. PubMed PMC

Delgado-Serra S, Sola J, Negre N and Paredes-Esquivel C (2022) Angiostrongylus cantonensis nematode invasion pathway, Mallorca, Spain. Emerging Infectious Diseases 28(6), 1163. PubMed PMC

Hidelaratchi M, Riffsy M and Wijesekera J (2010) A case of eosinophilic meningitis following monitor lizard meat consumption, exacerbated by anthelminthics. Ceylon Medical Journal 50(2). doi:10.4038/cmj.v50i2.1577 PubMed DOI

Jaume-Ramis S, Martínez-Ortí A, Delgado-Serra S, Bargues MD, MasComa S, Foronda P and Paredes-Esquivel C (2023) Potential intermediate hosts of Angiostrongylus cantonensis in the European Mediterranean region (Mallorca, Spain). One Health. 17, 100610. PubMed PMC

Kanpittaya J, Jitpimolmard S, Tiamkao S and Mairiang E (2000) MR findings of eosinophilic meningoencephalitis attributed to Angiostrongylus cantonensis. American Journal of Neuroradiology 21(6), 1090–1094. PubMed PMC

Kim D, Stewart T, Bauer R and Mitchell M (2002) Parastrongylus (= Angiostrongylus) cantonensis now endemic in Louisiana wildlife. Journal of Parasitology 88(5), 1024–1026. PubMed

Kim JR, Hayes KA, Yeung NW and Cowie RH (2014) Diverse gastropod hosts of Angiostrongylus cantonensis, the rat lungworm, globally and with a focus on the Hawaiian Islands. PLoS One 9(5), e94969. PubMed PMC

Lendrum AC, Fraser DS, Slidders W and Henderson R (1962) Studies on the character and staining of fibrin. Journal of Clinical Pathology 15(5), 401. PubMed PMC

Llewellyn BD (1970) An improved Sirius red method for amyloid. The Journal of Medical Laboratory Technology 27(3), 308–309. PubMed

Lopes-Torres EJ, de Oliveira Simões R, Mota EM and Thiengo SC (2024) A structural analysis of host–parasite interactions in Achatina fulica (Giant African snail) infected with Angiostrongylus cantonensis. Pathogens 13(1), 43. PubMed PMC

Martín-Carrillo N, Feliu C, Abreu-Acosta N, Izquierdo-Rodriguez E, Dorta-Guerra R, Miquel J, Abreu-Yanes E, Martin-Alonso A, García-Livia K, Quispe-Ricalde MA, et al. (2021) A peculiar distribution of the emerging nematode Angiostrongylus cantonensis in the Canary Islands (Spain): Recent introduction or isolation effect? Animals 11(5), 1267. PubMed PMC

Modrý D, Fecková B, Putnová B, Manalo SM and Otranto D (2021) Alternative pathways in Angiostrongylus cantonensis (Metastrongyloidea: Angiostrongylidae) transmission. Parasitology 148(2), 167–173. PubMed PMC

Monks DJ, Carlisle MS, Carrigan M, Rose K, Spratt D, Gallagher A and Prociv P (2005) Angiostrongylus cantonensis as a cause of cerebrospinal disease in a yellow tailed black cockatoo (Calyptorhynchus funereus) and two tawny frogmouths (Podargus strigoides). Journal of Avian Medicine and Surgery 19(4), 289–293.

Odening K (1976) Conception and terminology of hosts in parasitology. Advances in Parasitology 14, 1–93. PubMed

Pandian D, Najer T and Modrý D (2023) An overview of Angiostrongylus cantonensis (Nematoda: Angiostrongylidae), an emerging cause of human angiostrongylosis on the Indian subcontinent. Pathogens 12(6), 851. PubMed PMC

Price ER (2016) The physiology of lipid storage and use in reptiles. Biological Reviews 92(3), 1406–1426. doi:10.1111/brv.12288 PubMed DOI

Radomyos P, Tungtrongchitr A and Praewanich R (1992). Experimental infection of yellow tree monitor (Varanus bengalensis) and related species with Angiostrongylus cantonensis. Southeast Asian Journal of Tropical Medicine and Public Health. PubMed

Radomyos P, Tungtrongchitr A, Praewanich R, Khewwatchan P, Kantangkul T, Junlananto P and Ayudhya S (1994) Occurrence of the infective stage of Angiostrongylus cantonensis in the yellow tree monitor (Varanus bengalensis) in five Provinces of Thailand. The Southeast Asian Journal of Tropical Medicine and Public Health 25(3), 498–500. PubMed

Rollins RL, Cowie RH, Echaluse MV and Medeiros MC (2021) Host snail species exhibit differential Angiostrongylus cantonensis prevalence and infection intensity across an environmental gradient. Acta Tropica 216, 105824. PubMed

Sears WJ, Qvarnstrom Y, Dahlstrom E, Snook K, Kaluna L, Baláž V, Fecková B, Šlapeta J, Modrý D, Jarvi S, et al. (2021) AcanR3990 qPCR: A novel, highly sensitive, bioinformatically informed assay to detect Angiostrongylus cantonensis infections. Clinical Infectious Diseases 73(7), e1594–e1600. PubMed PMC

Spratt DM (2015) Species of Angiostrongylus (Nematoda: Metastrongyloidea) in wildlife: A review. International Journal for Parasitology: Parasites and Wildlife 4(2), 178–189. PubMed PMC

Turck HC, Fox MT and Cowie RH (2022). Paratenic hosts of Angiostrongylus cantonensis and their relation to human neuroangiostrongyliasis globally. One Health, 100426. PubMed PMC

Wallace GD and Rosen L (1967) Studies on eosinophilic meningitis. 4. Experimental infection of fresh-water and marine fish with Angiostrongylus cantonensis. American Journal of Epidemiology 85(3), 395–402. PubMed

Wallace GD, Rosen L, et al. (1966) Studies on eosinophilic meningitis. 2. Experimental infection of shrimp and crabs with Angiostrongylus cantonensis. American Journal of Epidemiology 84(1), 120–131. PubMed

Wangpan T and Tang Jang S (2012) Monitor lizards and geckos used in traditional medicine face extinction and need protection. Current Science 102(9), 1248.

Yang L, Darasavath C, Chang K, Vilay V, Sengduangphachanh A, Adsamouth A, Vongsouvath M, Keolouangkhot V and Robinson MT (2021) Cluster of angiostrongyliasis cases following consumption of raw monitor lizard in the Lao People’s Democratic Republic and review of the literature. Tropical Medicine and Infectious Disease 6(3), 107. PubMed PMC

Zaaf A, Herrel A, Aerts P and De Vree F (1999) Morphology and morphometrics of the appendicular musculature in geckoes with different locomotor habits (Lepidosauria). Zoomorphology 119(1), 9–22.