Altogether three species of Empidinae are described from San Rossore National Park, Italy: Empis (Euempis) sanrossorensis Barták sp. nov., Hilara polymorpha Barták sp. nov., and Rhamphomyia (Megacyttarus) sanrossorensis Barták sp. nov. Polymorphism in the shape of foreleg in Hilara is reported for the first time. The COI sequences for barcoding purposes and upcoming studies are provided.

Department of Agriculture Food Environment and Forestry University of Florence Piazzale delle Cascine 18 50144 Firenze Italy

Department of Botany and Zoology Faculty of Science Masaryk University Kotlářská 2 611 37 Brno Czech Republic

Department of Zoology and Fisheries Faculty of Agrobiology Food and Natural Resources Czech University of Life Sciences Prague Kamýcká 129 165 00 Praha Suchdol Czech Republic

VirNat s r o Na medzi 1C 83106 Bratislava Slovakia

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