Recent events involving nerve agents of the A-Series, a once elusive class of chemical warfare agents, have provoked a great concern in the international community. In this paper, continuing our research efforts in Medicinal Chemistry at the Brazilian Institute of Chemical, Biological, Radiological and Nuclear Defense (IDQBRN) (an OPCW-designated Laboratory for environmental samples), we explore ANMP, an A-230 surrogate, in the search for new treatment options for intoxications caused by these chemicals. Five isatin-pyridine oxime hybrids were evaluated as acetylcholinesterase (AChE) reactivators using a modified Ellman's assay. Our results indicate that monocationic hybrids with five methylene units, as well as its oxa-analog, are promising compounds for the design of new AChE reactivators.

Agency for Management and Technological Innovation Brazilian Army Technological Center Av das Américas 28705 Área 4 Rio de Janeiro RJ 23020 470 Brazil

Biomedical Research Centre University Hospital in Hradec Kralove Sokolska 581 50003 Hradec Kralove Czech Republic

Center for Advanced Innovation Technologies VSB Technical University of Ostrava 70800 Ostrava Poruba Ostrava Czech Republic

Center for Basic and Applied Research Faculty of Informatics and Management University of Hradec Kralove 50003 Hradec Kralove Czech Republic

Institute of Chemical Biological Radiological and Nuclear Defense Av das Américas 28705 Área 4 Rio de Janeiro RJ 23020 470 Brazil

Laboratory of Molecular Modeling Applied to the Chemical and Biological Defense Military Institute of Engineering Praca General Tiburcio 80 22290 270 Rio de Janeiro Brazil

Military Institute of Engineering Praça General Tibúrcio 80 Rio de Janeiro RJ 22290 270 Brazil

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