Polarity-guided uneven mitotic divisions control brassinosteroid activity in proliferating plant root cells
Language English Country United States Media print-electronic
Document type Journal Article
PubMed
40068682
DOI
10.1016/j.cell.2025.02.011
PII: S0092-8674(25)00196-5
Knihovny.cz E-resources
- Keywords
- brassinosteroids, cell cycle, cell division, cell polarity, live-cell imaging, root meristem, single-cell RNA sequencing,
- MeSH
- Arabidopsis * metabolism cytology growth & development MeSH
- Brassinosteroids * metabolism MeSH
- Plant Roots * cytology metabolism growth & development MeSH
- Meristem metabolism cytology MeSH
- Mitosis * MeSH
- Cell Polarity * MeSH
- Cell Proliferation MeSH
- Arabidopsis Proteins metabolism genetics MeSH
- Gene Expression Regulation, Plant MeSH
- Plant Growth Regulators metabolism MeSH
- Signal Transduction MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Brassinosteroids * MeSH
- Arabidopsis Proteins MeSH
- Plant Growth Regulators MeSH
Brassinosteroid hormones are positive regulators of plant organ growth, yet their function in proliferating tissues remains unclear. Here, through integrating single-cell RNA sequencing with long-term live-cell imaging of the Arabidopsis root, we reveal that brassinosteroid activity fluctuates throughout the cell cycle, decreasing during mitotic divisions and increasing during the G1 phase. The post-mitotic recovery of brassinosteroid activity is driven by the intrinsic polarity of the mother cell, resulting in one daughter cell with enhanced brassinosteroid signaling, while the other supports brassinosteroid biosynthesis. The coexistence of these distinct daughter cell states during the G1 phase circumvents a negative feedback loop to facilitate brassinosteroid production while signaling increases. Our findings uncover polarity-guided, uneven mitotic divisions in the meristem, which control brassinosteroid hormone activity to ensure optimal root growth.
Centro de Biotecnología y Genómica de Plantas Madrid 28040 Spain
Department of Biology Duke University Durham NC USA
Faculty of Biology Technion Israel Institute of Technology Haifa 3200003 Israel
Institute of Molecular Plant Biology Department of Biology ETH Zurich Zurich 8092 Switzerland
Laboratoire de Biogenèse Membranaire UMR5200 CNRS Université de Bordeaux Villenave d'Ornon France
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