Eight unprecedented terpenoids were isolated from submerged cultures of a polyporoid basidiomycete originating from Thailand (which had been referred to as "Cerrena sp." in a previous publication) by preparative chromatography. Their chemical structures were elucidated by extensive two-dimensional nuclear magnetic resonance (NMR) spectroscopy and high-resolution mass spectrometry. One of the compounds was crystallized, and its absolute configuration was established by X-ray crystallography. Among the isolated metabolites were several members of the rare nitrogen-containing drimane type and one dimeric drimane, which consists of a nitrogen-containing monomer and a regular monomer. The latter compound represents a hitherto unknown type of terpenoid natural product. The metabolites were subjected to a biological characterization, and some of them showed significant neurotrophic effects. Notably, several of the compounds significantly enhanced the outgrowth of neurites in PC12 cells when treated with 5 ng/mL nerve growth factor. On the other hand, they were devoid of significant cytotoxic and antimicrobial effects.

Department of Biotechnology Faculty of Science Mahidol University Bangkok 10400 Thailand

Department of Cell Biology Helmholtz Centre for Infection Research Inhoffenstrasse 7 38124 Braunschweig Germany

Department of Inorganic Chemistry Charles University Hlavova 2030 8 CZ 128 00 Prague 2 Czech Republic

Department of Microbial Drugs Helmholtz Centre for Infection Research Inhoffenstrasse 7 38124 Braunschweig Germany

Institute of Microbiology Technische Universität Braunschweig Spielmannstraße 7 38106 Braunschweig Germany

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