Electron Microscopy of Human Oocytes
Language English Country United States Media print
Document type Journal Article
- Keywords
- Electron microscopy, Focused ion beam scanning electron microscopy, Human oocytes, Transmission electron microscopy, Ultrastructure,
- MeSH
- Microscopy, Electron * methods MeSH
- Humans MeSH
- Oocytes * ultrastructure MeSH
- Microscopy, Electron, Transmission methods MeSH
- Check Tag
- Humans MeSH
- Female MeSH
- Publication type
- Journal Article MeSH
Electron microscopy represents a powerful visualizing technique, capable of a million times magnification. Prior to imaging, biological samples must undergo complex preparation to withstand the exposition to electrons in the vacuum inside the electron microscope. Here, we describe a preparation technique allowing preservation of scarce and delicate human oocytes for ultrastructural investigation.
Faculty of Medicine Department of Histology and Embryology Masaryk University Brno Czech Republic
Reprofit International Clinic of Reproductive Medicine Brno Czech Republic
See more in PubMed
Garza-Lopez E, Vue Z, Katti P et al (2021) Protocols for generating surfaces and measuring 3D organelle morphology using Amira. Cells 11(1):65
Trebichalská Z, Kyjovská D, Kloudová S et al (2021) Cytoplasmic maturation in human oocytes: an ultrastructural study. Biol Reprod 104(1):106–116
Trebichalská Z, Javůrek J, Tatíčková M et al (2021) High-resolution 3D reconstruction of human oocytes using focused ion beam scanning electron microscopy. Front Cell Dev Biol 9:755740
Tatíčková M, Trebichalská Z, Kyjovská D et al (2023) The ultrastructural nature of human oocytes’ cytoplasmic abnormalities and the role of cytoskeleton dysfunction. F S Sci 4(4):267–278
Hyttel P, Madsen I (1987) Rapid method to prepare mammalian oocytes and embryos for transmission electron microscopy. Acta Anat 129(1):12–14
Schiff RI, Gennaro JF (1979) The role of the buffer in the fixation of biological specimens for transmission and scanning electron microscopy. Scanning 2:135–148
Mizihura V, Futaesaku Y (1972) New fixation for biological membranes using tannic acids. Acta Histochem Cytochem 5(4):233–236
Porter KR, Kallman F (1953) The properties and effects of osmium tetroxide as a tissue fixative with special reference to its use for electron microscopy. Exp Cell Res 4(1):127–141
Aguas AP (1982) The use of osmium tetroxide-potassium ferrocyanide as an extracellular tracer in electron microscopy. Stain Technol 57(2):69–73
Ellis EA (2014) Staining sectioned biological specimens for transmission electron microscopy: conventional and en bloc stains. Methods Mol Biol 1117:57–72
