Fed-batch is the most commonly used cultivation mode for industrial production of recombinant proteins with Pichia pastoris. On a laboratory scale, fed-batch culture provides a way to control the specific biomass growth rates at any pre-set value, allowing the conditions of biomass growth and recombinant product formation to be systematically studied.In this chapter, we present an accessible and versatile approach for designing, performing, and evaluating a fed-batch cultivation in laboratory-scale stirred tank bioreactors.

Department of Biotechnology University of Chemistry and Technology Prague Dejvice Czech Republic

Infors HT Bottmingen Switzerland

Molecular Partners AG Schlieren Switzerland

spool Wädenswil Switzerland

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