A Guide to Fed-Batch Cultivation of Pichia pastoris on Laboratory Scale
Jazyk angličtina Země Spojené státy americké Médium print
Typ dokumentu časopisecké články
- Klíčová slova
- Bioprocess design and development, Bioreactor, Data evaluation, Fed-batch culture, Feed rate, Fermentation, Komagataella phaffii, Pichia pastoris, Specific growth rate, Specific productivity,
- MeSH
- biomasa MeSH
- bioreaktory mikrobiologie MeSH
- fermentace MeSH
- kultivační média MeSH
- Pichia * růst a vývoj MeSH
- rekombinantní proteiny genetika biosyntéza MeSH
- Saccharomycetales * růst a vývoj metabolismus MeSH
- techniky vsádkové kultivace * metody MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- kultivační média MeSH
- rekombinantní proteiny MeSH
Fed-batch is the most commonly used cultivation mode for industrial production of recombinant proteins with Pichia pastoris. On a laboratory scale, fed-batch culture provides a way to control the specific biomass growth rates at any pre-set value, allowing the conditions of biomass growth and recombinant product formation to be systematically studied.In this chapter, we present an accessible and versatile approach for designing, performing, and evaluating a fed-batch cultivation in laboratory-scale stirred tank bioreactors.
Department of Biotechnology University of Chemistry and Technology Prague Dejvice Czech Republic
Infors HT Bottmingen Switzerland
Zobrazit více v PubMed
Meyer H, Schmidhalter DR (2014) Industrial scale suspension culture of living cells. Wiley-VCH Verlag GmbH & Co KGaA. https://doi.org/10.1002/9783527683321 DOI
Looser V, Brühlmann B, Bumbak F et al (2015) Cultivation strategies to enhance productivity of Pichia pastoris: a review. Biotechnol Adv 33:1177–1193. https://doi.org/10.1016/j.biotechadv.2015.05.008 PubMed DOI
Potgieter TI, Kersey SD, Mallem MR et al (2010) Antibody expression kinetics in glycoengineered Pichia pastoris. Biotechnol Bioeng 106:918–927. https://doi.org/10.1002/bit.22756 PubMed DOI
Hyka P, Züllig T, Ruth C et al (2010) Combined use of fluorescent dyes and flow cytometry to quantify the physiological state of Pichia pastoris during the production of heterologous proteins in high-cell-density fed-batch cultures. Appl Environ Microbiol 76:4486–4496. https://doi.org/10.1128/aem.02475-09 PubMed DOI PMC
