We present a genome assembly from a female specimen of Spialia sertorius (Red-underwing Skipper; Arthropoda; Insecta; Lepidoptera; Hesperiidae). The assembly contains two haplotypes with total lengths of 364.57 megabases and 323.91 megabases. Most of haplotype 1 (99.92%) is scaffolded into 32 chromosomal pseudomolecules, including the W and Z sex chromosomes. Haplotype 2 was assembled to scaffold level. The mitochondrial genome has also been assembled, with a length of 15.34 kilobases.

Biology Centre of the Academy of Sciences of the Czech Republic Senckenberg German Entomological Institute South Bohemian University in Ceske Budjeovice Ceske Budjeovice Czech Republic

Tree of Life Wellcome Sanger Institute Hinxton England UK

University of Neuchâtel Neuchâtel Switzerland

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