This study examines the decontamination efficacy of cold atmospheric plasma (CAP) against Staphylococcus epidermidis and Escherichia coli, with emphasis on surface scanning parameters and resistance potential. Bacterial cultures on solid nutrient media were treated using a non-thermal microwave plasma torch operated with high-purity argon (4.6) at 5 Slm and 12-13 W. Treatment was systematically varied in scanning direction, speed, and duration. Results indicate that exposure time was the main factor influencing bacterial inhibition, whereas direction and speed exerted only minor effects. E. coli exhibited greater susceptibility compared to S. epidermidis. Repeated exposure experiments revealed no evidence of resistance development.

Faculty of Chemistry Brno University of Technology Purkyňova 118 612 00 Brno Czech Republic

University of Chemistry and Technology Prague Technická 5 Dejvice 166 28 Praha 6 Czech Republic

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