Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake.

Annotation

flow cell sorting & FLUORESCENCE-ACTIVATED CELL SORTING: coord with CELL SEPARATION

DUI

D005434 MeSH Browser

CUI

M0008573

History note

82; FLOW MICROFLUORIMETRY was see FLUOROIMMUNOASSAY 1989-90

Online note

use FLUOROIMMUNOASSAY to search FLOW MICROFLUORIMETRY 1989-90

Public note

82; FLOW MICROFLUORIMETRY was see FLUOROIMMUNOASSAY 1989-90