Negative Staining [negativní barvení]

topical
1
Persistent link   https://www.medvik.cz/link/D016624
Definition

The technique of washing tissue specimens with a concentrated solution of a heavy metal salt and letting it dry. The specimen will be covered with a very thin layer of the metal salt, being excluded in areas where an adsorbed macromolecule is present. The macromolecules allow electrons from the beam of an electron microscope to pass much more readily than the heavy metal; thus, a reversed or negative image of the molecule is created.

DUI
D016624 MeSH Browser
CUI
M0025341
Previous indexing
Staining (1966-1991)
History note
92
Public note
92

Allowable subheadings

CL
classification
EC
economics
ES
ethics
HI
history
IS
instrumentation
MT
methods
ST
standards
SN
statistics & numerical data
TD
trends
VE
veterinary

E Analytical, Diagnostic and Therapeutic Techniques and Equipment
E01 Diagnosis 1 790
E01.370 Diagnostic Techniques and Procedures 3 418
E01.370.225 Clinical Laboratory Techniques 6 378
E01.370.225.500 Cytological Techniques 563
E01.370.225.500.620 Histocytological Preparation Techniques 56
E01.370.225.500.620.670 Staining and Labeling 573
E01.370.225.500.620.670.130 Chromosome Banding 55
E01.370.225.500.620.670.325 In Situ Hybridization 159
E01.370.225.500.620.670.520 Negative Staining 1
E01.370.225.500.620.670.570 Neuroanatomical Tract-Tracing Techniques 1
E01.370.225.500.620.670.615 Periodic Acid-Schiff Reaction 5
E01.370.225.500.620.670.660 Prussian Blue Reaction
E01.370.225.500.620.670.770 Shadowing Technique, Histology 24
E01.370.225.500.620.670.780 Silver Staining 24
E01.370.225.750 Histological Techniques 1 391
E01.370.225.750.600 Histocytological Preparation Techniques 56
E01.370.225.750.600.670 Staining and Labeling 573
E01.370.225.750.600.670.130 Chromosome Banding 55
E01.370.225.750.600.670.325 In Situ Hybridization 159
E01.370.225.750.600.670.520 Negative Staining 1
E01.370.225.750.600.670.570 Neuroanatomical Tract-Tracing Techniques 1
E01.370.225.750.600.670.660 Prussian Blue Reaction
E01.370.225.750.600.670.770 Shadowing Technique, Histology 24
E01.370.225.750.600.670.780 Silver Staining 24
E05 Investigative Techniques 209
E05.200 Clinical Laboratory Techniques 6 378
E05.200.500 Cytological Techniques 563
E05.200.500.620 Histocytological Preparation Techniques 56
E05.200.500.620.670 Staining and Labeling 573
E05.200.500.620.670.130 Chromosome Banding 55
E05.200.500.620.670.325 In Situ Hybridization 159
E05.200.500.620.670.520 Negative Staining 1
E05.200.500.620.670.570 Neuroanatomical Tract-Tracing Techniques 1
E05.200.500.620.670.620 Periodic Acid-Schiff Reaction 5
E05.200.500.620.670.660 Prussian Blue Reaction
E05.200.500.620.670.770 Shadowing Technique, Histology 24
E05.200.500.620.670.780 Silver Staining 24
E05.200.750 Histological Techniques 1 391
E05.200.750.600 Histocytological Preparation Techniques 56
E05.200.750.600.670 Staining and Labeling 573
E05.200.750.600.670.130 Chromosome Banding 55
E05.200.750.600.670.325 In Situ Hybridization 159
E05.200.750.600.670.520 Negative Staining 1
E05.200.750.600.670.570 Neuroanatomical Tract-Tracing Techniques 1
E05.200.750.600.670.660 Prussian Blue Reaction
E05.200.750.600.670.770 Shadowing Technique, Histology 24
E05.200.750.600.670.780 Silver Staining 24

Add