Reverse Transcriptase Polymerase Chain Reaction [polymerázová řetězová reakce s reverzní transkripcí]

topical
704
Terms

PCR spojená s reverzní transkripcí
reverzní PCR
RT-PCR

 

Polymerase Chain Reaction, Reverse Transcriptase
Reverse Transcriptase PCR

Persistent link   https://www.medvik.cz/link/D020133
Definition

A variation of the PCR technique in which cDNA is made from RNA via reverse transcription. The resultant cDNA is then amplified using standard PCR protocols.

Annotation
RT-PCR in the literature probably goes here not under REAL-TIME POLYMERASE CHAIN REACTION; check text
DUI
D020133 MeSH Browser
CUI
M0029872
Previous indexing
Polymerase Chain Reaction (1991-1998); RNA-Directed DNA Polymerase (1991-1998)
History note
1999
Public note
1999

Allowable subheadings

CL
classification
EC
economics 1
ES
ethics
HI
history
IS
instrumentation 10
MT
methods 162
ST
standards 10
SN
statistics & numerical data 3
TD
trends 1
VE
veterinary 3

E Analytical, Diagnostic and Therapeutic Techniques and Equipment
E01 Diagnosis 1 790
E02 Therapeutics 2 575
E03 Anesthesia and Analgesia 168
E04 Surgical Procedures, Operative 4 389
E05 Investigative Techniques 209
E05.393 Genetic Techniques 579
E05.393.620 Nucleic Acid Amplification Techniques 121
E05.393.620.500 Polymerase Chain Reaction 2 410
E05.393.620.500.324 Amplified Fragment Length Polymorphism Analysis 37
E05.393.620.500.487 Multiplex Polymerase Chain Reaction 69
E05.393.620.500.650 Primed In Situ Labeling 13
E05.393.620.500.687 Random Amplified Polymorphic DNA Technique 59
E05.393.620.500.706 Real-Time Polymerase Chain Reaction 510
E05.393.620.500.725 Reverse Transcriptase Polymerase Chain Reaction 704
E06 Dentistry 1 670
E07 Equipment and Supplies 4 036

Add