Nuclease Protection Assays [testy rezistence k nukleáze]

topical
1
Persistent link   https://www.medvik.cz/link/D023282
Definition

Techniques for measuring specific nucleic acid interaction with another nucleic acid or with a protein by digestion of the non-interacting nucleic acid by various nucleases. After all non-interacting regions are eliminated by nuclease digestion, the protected nucleic acid that remains is analyzed. DNA FOOTPRINTING utilizes this technique to analyze the DNA contact sites of DNA-BINDING PROTEINS.

DUI
D023282 MeSH Browser
CUI
M0361086
History note
2001
Public note
2001

Allowable subheadings

CL
classification
EC
economics
ES
ethics
HI
history
IS
instrumentation
MT
methods
ST
standards
SN
statistics & numerical data
TD
trends
VE
veterinary

E Analytical, Diagnostic and Therapeutic Techniques and Equipment
E01 Diagnosis 1 790
E02 Therapeutics 2 575
E03 Anesthesia and Analgesia 168
E04 Surgical Procedures, Operative 4 389
E05 Investigative Techniques 209
E05.393 Genetic Techniques 579
E05.393.085 Cellular Reprogramming Techniques 5
E05.393.128 Chemogenetics
E05.393.170 Chromatin Immunoprecipitation 32
E05.393.183 Chromosome Mapping 425
E05.393.220 Cloning, Molecular 406
E05.393.240 Cloning, Organism 97
E05.393.260 Contig Mapping 9
E05.393.281 Crosses, Genetic 96
E05.393.285 Cytogenetic Analysis 602
E05.393.290 DNA Fingerprinting 138
E05.393.300 DNA Footprinting 12
E05.393.332 Gene Expression Profiling 798
E05.393.335 Gene Targeting 28
E05.393.350 Gene Transfer Techniques 86
E05.393.385 Genetic Association Studies 267
E05.393.420 Genetic Engineering 520
E05.393.435 Genetic Testing 1 263
E05.393.442 Genotyping Techniques 161
E05.393.475 In Situ Nick-End Labeling 36
E05.393.520 Molecular Diagnostic Techniques 1 025
E05.393.522 Molecular Epidemiology 148
E05.393.542 Molecular Typing 96
E05.393.560 Mutagenicity Tests 441
E05.393.600 Nuclease Protection Assays 1
E05.393.620 Nucleic Acid Amplification Techniques 121
E05.393.640 Nucleic Acid Denaturation 62
E05.393.661 Nucleic Acid Hybridization 171
E05.393.667 Optogenetics 15
E05.393.673 Pedigree 546
E05.393.712 Restriction Mapping 65
E05.393.731 Reverse Genetics 4
E05.393.751 Sequence Alignment 301
E05.393.760 Sequence Analysis 135
E05.393.880 Viral Pseudotyping 1
E05.393.940 Shotgun Sequencing
E06 Dentistry 1 670
E07 Equipment and Supplies 4 036

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