A technique for identifying specific DNA sequences that are bound, in vivo, to proteins of interest. It involves formaldehyde fixation of CHROMATIN to crosslink the DNA-BINDING PROTEINS to the DNA. After shearing the DNA into small fragments, specific DNA-protein complexes are isolated by immunoprecipitation with protein-specific ANTIBODIES. Then, the DNA isolated from the complex can be identified by PCR amplification and sequencing.

DUI

D047369 MeSH Prohlížeč

CUI

M0457418

Předchozí užití

Precipitin Tests (2000-2004)

Historická pozn.

2005

Veřejná pozn.

2005