Chromatin Immunoprecipitation [chromatinová imunoprecipitace]

topical
32
Terms

chromatin - imunoprecipitace

 

Immunoprecipitation, Chromatin

Persistent link   https://www.medvik.cz/link/D047369
Definition

A technique for identifying specific DNA sequences that are bound, in vivo, to proteins of interest. It involves formaldehyde fixation of CHROMATIN to crosslink the DNA-BINDING PROTEINS to the DNA. After shearing the DNA into small fragments, specific DNA-protein complexes are isolated by immunoprecipitation with protein-specific ANTIBODIES. Then, the DNA isolated from the complex can be identified by PCR amplification and sequencing.

DUI
D047369 MeSH Browser
CUI
M0457418
Previous indexing
Precipitin Tests (2000-2004)
History note
2005
Public note
2005

Allowable subheadings

AE
adverse effects
CL
classification
EC
economics
ES
ethics
HI
history
IS
instrumentation
MT
methods 4
MO
mortality
NU
nursing
PX
psychology
ST
standards
SN
statistics & numerical data
TD
trends
VE
veterinary

E Analytical, Diagnostic and Therapeutic Techniques and Equipment
E05 Investigative Techniques 209
E05.393 Genetic Techniques 581
E05.393.085 Cellular Reprogramming Techniques 5
E05.393.128 Chemogenetics
E05.393.170 Chromatin Immunoprecipitation 32
E05.393.170.500 Chromatin Immunoprecipitation Sequencing 2
E05.393.183 Chromosome Mapping 427
E05.393.220 Cloning, Molecular 407
E05.393.240 Cloning, Organism 98
E05.393.260 Contig Mapping 9
E05.393.281 Crosses, Genetic 96
E05.393.285 Cytogenetic Analysis 602
E05.393.290 DNA Fingerprinting 139
E05.393.300 DNA Footprinting 12
E05.393.332 Gene Expression Profiling 809
E05.393.335 Gene Targeting 28
E05.393.350 Gene Transfer Techniques 86
E05.393.385 Genetic Association Studies 270
E05.393.420 Genetic Engineering 520
E05.393.435 Genetic Testing 1 289
E05.393.442 Genotyping Techniques 161
E05.393.475 In Situ Nick-End Labeling 36
E05.393.520 Molecular Diagnostic Techniques 1 034
E05.393.522 Molecular Epidemiology 148
E05.393.542 Molecular Typing 96
E05.393.560 Mutagenicity Tests 441
E05.393.600 Nuclease Protection Assays 1
E05.393.620 Nucleic Acid Amplification Techniques 123
E05.393.640 Nucleic Acid Denaturation 62
E05.393.661 Nucleic Acid Hybridization 172
E05.393.667 Optogenetics 15
E05.393.673 Pedigree 550
E05.393.712 Restriction Mapping 65
E05.393.731 Reverse Genetics 4
E05.393.751 Sequence Alignment 301
E05.393.760 Sequence Analysis 136
E05.393.880 Viral Pseudotyping 1
E05.393.940 Shotgun Sequencing
E05.478 Immunologic Techniques 1 014
E05.478.605 Immunoprecipitation 57
E05.478.605.160 Chromatin Immunoprecipitation 32
E05.478.605.160.500 Chromatin Immunoprecipitation Sequencing 2
E05.478.605.492 Precipitin Tests 55
E05.478.605.825 Radioimmunoprecipitation Assay 12

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