Fluorescence microscopy utilizing multiple low-energy photons to produce the excitation event of the fluorophore (endogenous fluorescent molecules in living tissues or FLUORESCENT DYES). Multiphoton microscopes have a simplified optical path in the emission side due to the lack of an emission pinhole, which is necessary with normal confocal microscopes. Ultimately this allows spatial isolation of the excitation event, enabling deeper imaging into optically thick tissue, while restricting photobleaching and phototoxicity to the area being imaged.

DUI

D036641 MeSH Browser

CUI

M0410252

Previous indexing

Microscopy, Confocal (1997-2002); Microscopy, Fluorescence (1996-2002)

History note

2003

Public note

2003