A diagnostics of infectious diseases can be done by the immunologic methods or by the amplification of nucleic acid specific to contagious agent using polymerase chain reaction. However, in transmissible spongiform encephalopathies, the infectious agent, prion protein (PrP(Sc)), has the same sequence of nucleic acids as a naturally occurring protein. The other issue with the diagnosing based on the PrP(Sc) detection is that the pathological form of prion protein is abundant only at late stages of the disease in a brain. Therefore, the diagnostics of prion protein caused diseases represent a sort of challenges as that hosts can incubate infectious prion proteins for many months or even years. Therefore, new in vivo assays for detection of prion proteins and for diagnosis of their relation to neurodegenerative diseases are summarized. Their applicability and future prospects in this field are discussed with particular aim at using quantum dots as fluorescent labels.
- MeSH
- fluorescenční barviva diagnostické užití MeSH
- kvantové tečky diagnostické užití MeSH
- lidé MeSH
- molekulární modely MeSH
- mozek patologie MeSH
- neurodegenerativní nemoci diagnóza patologie MeSH
- prionové nemoci diagnóza patologie MeSH
- priony analýza MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- přehledy MeSH
- MeSH
- algoritmy MeSH
- financování organizované MeSH
- intrakraniální subdurální hematom diagnóza MeSH
- lidé MeSH
- magnetická rezonanční tomografie metody využití MeSH
- Markovovy řetězce MeSH
- počítačová rentgenová tomografie metody využití MeSH
- počítačové zpracování obrazu MeSH
- teoretické modely MeSH
- Check Tag
- lidé MeSH
