We investigated the expression of vascular endothelial growth factor (VEGF) and its receptors (Flt-1 and KDR/Flk-1) during muscle regeneration by immunohistochemistry and real-time RT-PCR. On days 5 and 7 after the induction of injury, VEGF and Flt-1 were detected in the cytoplasm and KDR/Flk-1 in the cytoplasm and on cell membranes of the same regenerating muscle fibers. The levels of these proteins in the regenerating muscle fibers gradually decreased until day 20. In contrast, these proteins were not detected in the fibers of normal muscle. This suggests that regenerating muscle fibers express VEGF and its receptors in response to injury. In addition, we found that the VEGF mRNA transcript transiently increased after 12 h of muscle injury and then returned to the basal levels observed in normal muscles on day 1. The expression of Flt-1 and KDR/Flk-1 mRNA transcripts also peaked on day 3 and then returned to the basal levels observed in normal muscles on day 10. These findings suggest that regenerating muscle fibers are an important source of VEGF and that VEGF signaling through Flt-1 and KDR/Flk-1 may be involved in the process of muscle regeneration in vivo.

• MeSH
• exprese genu fyziologie   genetika   MeSH
• financování vládou MeSH
• imunohistochemie metody   využití   MeSH
• interpretace statistických dat MeSH
• kosterní svaly fyziologie   růst a vývoj   MeSH
• myši anatomie a histologie   fyziologie   růst a vývoj   MeSH
• polymerázová řetězová reakce s reverzní transkripcí metody   využití   MeSH
• receptory vaskulárního endoteliálního růstového faktoru genetika   MeSH
• vaskulární endoteliální růstový faktor A fyziologie   MeSH
• zvířata MeSH
• Check Tag
• myši anatomie a histologie   fyziologie   růst a vývoj   MeSH
• zvířata MeSH
• Publikační typ
• srovnávací studie MeSH