The fast and efficient detection of foodborne pathogens is a societal priority, given the large number of food-poisoning outbreaks, and a scientific and technological challenge, given the need to detect as little as 1 viable cell in 25 gr of food. Here, we present the first approach that achieves the above goal, thanks to the use of a micro/nano-technology and the detection capability of acoustic wave sensors. Starting from 1 Salmonella cell in 25 ml of milk, we employ immuno-magnetic beads to capture cells after only 3 h of pre-enrichment and subsequently demonstrate efficient DNA amplification using the Loop Mediated Isothermal Amplification method (LAMP) and acoustic detection in an integrated platform, within an additional ½ h. The demonstrated 4 h sample-to-analysis time comes as a huge improvement to the current need of few days to obtain the same result. In addition, the work presents the first reported Lab-on-Chip platform that comprises an acoustic device as the sensing element, exhibiting impressive analytical features, namely, an acoustic limit of detection of 2 cells/μl or 3 aM of the DNA target and ability to detect in a label-free manner dsDNA amplicons in impure samples. The use of food samples together with the incorporation of the necessary pre-enrichment step and ability for multiple analysis with an internal control, make the proposed methodology highly relevant to real-world applications. Moreover, the work suggests that acoustic wave devices can be used as an attractive alternative to electrochemical sensors in integrated platforms for applications in food safety and the point-of-care diagnostics.

• MeSH
• akustika přístrojové vybavení   MeSH
• analýza potravin přístrojové vybavení   MeSH
• biosenzitivní techniky přístrojové vybavení   MeSH
• design vybavení MeSH
• DNA bakterií analýza   genetika   MeSH
• kontaminace potravin analýza   MeSH
• laboratoř na čipu MeSH
• lidé MeSH
• limita detekce MeSH
• mléko mikrobiologie   MeSH
• nemoci přenášené potravou mikrobiologie   MeSH
• potravinářská mikrobiologie MeSH
• Salmonella genetika   izolace a purifikace   MeSH
• salmonelóza mikrobiologie   MeSH
• zvířata MeSH
• zvuk MeSH
• Check Tag
• lidé MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• hodnotící studie MeSH