* Zobrazit nápovědu

CEVJ. 2011 ; 10 (1) : 8-9. (XXXVI Angiologicial Days 2011 with International Participation. Annual Conference of the Czech Society of Angiology. Prague, February 24-26, 2011)

CEVJ (Praha)
ISSN 1214-3901
Medvik
Zdroj

Angiological Days ... with international participation. 2011 ; () : .

Medvik
Zdroj

Publikační typ
abstrakt z konference MeSH

Abstrakt

Genetic predisposition to sepsis in children: the combination of 5 risk gene variants predicts outcome of life- threatening sepsis

Analytical Cytometry. 2011 ; () : 91-92.

ISBN 978-80-260-0676-3
Medvik
Zdroj

Publikační typ
abstrakt z konference MeSH

Článek

The early effect of dextran sodium sulfate administration on carbachol-induced short-circuit current in distal and proximal colon during colitis development

Physiological research. 2011 ; 60 (6) : 921-931.

Physiol. Res. (Print)
ISSN 0862-8408
Medvik
Zdroj

Increased colonic Cl- secretion was supposed to be a causative factor of diarrhea in inflammatory bowel diseases. Surprisingly, hyporesponsiveness to Cl- secretagogues was later described in inflamed colon. Our aim was to evaluate changes in secretory responses to cholinergic agonist carbachol in distal and proximal colon during colitis development, regarding secretory activity of enteric nervous system (ENS) and prostaglandins. Increased responsiveness to carbachol was observed in both distal and proximal colon after 3 days of 2 % dextran sodium sulfate (DSS) administration. It was measured in the presence of mucosal Ba2+ to emphasize Cl- secretion. The described increase was abolished by combined inhibitory effect of tetrodotoxin (TTX) and indomethacin. Indomethacin also significantly reduced TTXsensitive current. On the 7th day of colitis development responsiveness to carbachol decreased in distal colon (compared to untreated mice), but did not change in proximal colon. TTXsensitive current did not change during colitis development, but indomethacin-sensitive current was significantly increased the 7th day. Decreased and deformed current responses to serosal Ba2+ were observed during colitis induction, but only in proximal colon. We conclude that besides inhibitory effect of DSS on distal colon responsiveness, there is an early stimulatory effect that manifests in both distal and proximal colon.

MeSH
karbachol MeSH
kolitida chemicky indukované patologie MeSH
kolon patofyziologie patologie účinky léků MeSH
myši inbrední BALB C MeSH
myši MeSH
prostaglandiny fyziologie MeSH
síran dextranu aplikace a dávkování farmakologie MeSH
střevní nervový systém patofyziologie MeSH
střevní sliznice patofyziologie patologie účinky léků MeSH
zvířata MeSH
Check Tag
mužské pohlaví MeSH
myši MeSH
zvířata MeSH
Publikační typ
práce podpořená grantem MeSH

Článek

Why we are not able to find the coronary heart disease gene - apoE as an example

Folia biologica (Praha). 2010 ; 56 (5) : 218-222.

Medvik
Zdroj

The apoprotein E gene ranks among the most discussed candidate genes for cardiovascular disease. We studied whether the association between apoprotein E gene polymorphism and manifestation of acute coronary syndrome is modulated by the presence/absence of traditional cardiovascular risk factors. The population under study were 1066 patients (men under 65 years) admitted between 2006- 2009 to five coronary care units in Prague (GENetic DEtermination of Myocardial Infarction in Prague) and the control population (1066 age-matched men selected from the Czech population sample). The frequency of disadvantage genotype E4+ was significantly higher (P < 0.01) in acute coronary syndrome patients (22.38 %) than in controls (16.76 %). When the acute coronary syndrome group was step by step limited to non-smokers, non-diabetics and normotensive individuals, the odds ratio displayed a gradual increase from 1.35 (for the entire group) through 1.48 (non-smokers), 1.53 (non-smokers+non-diabetics) to 1.71 (non-smokers+non-diabetics+normotensives). The effect of the apoprotein E gene on the individual risk of acute coronary syndrome is nonhomogenous within the patient groups. This association of apoprotein E gene with acute coronary syndrome is strongly modified by the presence/absence of traditional cardiovascular factors of atherosclerosis in a high-risk Czech population.

Článek

Acute and delayed implantation of positively charged 2-hydroxyethyl methacrylate scaffolds in spinal cord injury in the rat

Journal of neurosurgery. Spine. 2008 ; 8 (1) : 67-73.

J Neurosurg Spine
ISSN 1547-5654
Medvik
Zdroj

OBJECT: Hydrogels are nontoxic, chemically inert synthetic polymers with a high water content and large surface area that provide mechanical support for cells and axons when implanted into spinal cord tissue. METHODS: Macroporous hydrogels based on 2-hydroxyethyl methacrylate (HEMA) were prepared by radical copolymerization of monomers in the presence of fractionated NaCl particles. Male Wistar rats underwent complete spinal cord transection at the T-9 level. To bridge the lesion, positively charged HEMA hydrogels were implanted either immediately or 1 week after spinal cord transection; control animals were left untreated. Histological evaluation was performed 3 months after spinal cord transection to measure the volume of the pseudocyst cavities and the ingrowth of tissue elements into the hydrogels. RESULTS: The hydrogel implants adhered well to the spinal cord tissue. Histological evaluation showed ingrowth of connective tissue elements, blood vessels, neurofilaments, and Schwann cells into the hydrogels. Morphometric analysis of lesions showed a statistically significant reduction in pseudocyst volume in the treated animals compared with controls and in the delayed treatment group compared with the immediate treatment group (p < 0.001 and p < 0.05, respectively). CONCLUSIONS: Positively charged HEMA hydrogels can bridge a posttraumatic spinal cord cavity and provide a scaffold for the ingrowth of regenerating axons. The results indicate that delayed implantation can be more effective than immediate reconstructive surgery.